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Kinetic profile and urinary excretion of phenyl-γ-valerolactones upon consumption of cranberry: a dose–response relationship

Favari, Claudia, Mena, Pedro, Curti, Claudio, Istas, Geoffrey, Heiss, Christian, Del Rio, Daniele and Rodriguez-Mateos, Ana (2020) Kinetic profile and urinary excretion of phenyl-γ-valerolactones upon consumption of cranberry: a dose–response relationship Food & Function, 11 (5). pp. 3975-3985.

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Cranberries are a rich source of poly(phenols), mainly monomeric and oligomeric flavan-3-ols. However, information on the appearance of their main circulating microbial metabolites, namely phenyl-γ-valerolactones and phenylvaleric acid, is lacking despite its relevance to understanding the health effects attributed to cranberries. The aim of this study was to evaluate the absorption, metabolism and urinary excretion of cranberry flavan-3-ols through the targeted analysis of phenyl-γ-valerolactones and their related phenylvaleric acids, considering also their potential as biomarkers of flavan-3-ol intake and inter-individual variability in their appearance in plasma and urine. A six-arm acute crossover, randomized, double-blinded, controlled intervention trial was performed in ten healthy males who consumed a cranberry juice drink (375, 716, 1131, 1396, 1741 mg of total flavan-3-ols) or an isocaloric control drink with one-week washout. Plasma and urine were analyzed by UHPLC-ESI-QqQ-MS/MS and 22 compounds were identified. Glucuronide and sulfate conjugates of 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone were the main circulating and excreted metabolites after cranberry juice intake, with glucuronidation appearing to be the most favorable conjugation route. These compounds reached maximum plasma concentration at about 4–6 h. Plasma and urinary concentrations of the sum of the metabolites increased in relation to the amounts of cranberry flavan-3-ols provided by the drink, showing a clear and linear dose-dependent relationship and underscoring their potential as biomarkers of flavan-3-ol intake. A high inter-individual variability in circulating and urinary metabolite levels was observed and, interestingly, some subjects seemed to display a greater efficiency in metabolizing flavan-3-ols and producing phenyl-γ-valerolactones.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Favari, Claudia
Mena, Pedro
Curti, Claudio
Istas, Geoffrey
Del Rio, Daniele
Date : May 2020
DOI : 10.1039/D0FO00806K
Copyright Disclaimer : © Royal Society of Chemistry 2020
Uncontrolled Keywords : Cranberry; Phenolic metabolites; Flavan-3-ols; Proanthocyanins; Phenyl-γ-valerolactones
Depositing User : Clive Harris
Date Deposited : 10 Jun 2020 11:58
Last Modified : 10 Jun 2020 11:58

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