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Platelet-derived Transforming Growth Factor-β1 promotes keratinocyte proliferation in cutaneous wound healing

Chong, Deborah L.H, Trinder, Sarah, Labelle, Myriam, Rodriguez-Justo, Manuel, Hughes, Sian, Holmes, Alan M., Scotton, Chris J. and Porter, Joanna C. (2020) Platelet-derived Transforming Growth Factor-β1 promotes keratinocyte proliferation in cutaneous wound healing Journal of Tissue Engineering and Regenerative Medicine.

Chong_et_al-2020-Journal_of_Tissue_Engineering_and_Regenerative_Medicine.pdf - Accepted version Manuscript

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Platelets are a recognised potent source of Transforming Growth Factor-1 (TGF1), a cytokine known to promote wound healingand regenerationby stimulating dermal fibroblast proliferation and extracellular matrix deposition. Platelet lysate has been advocated as a novel personalised therapeutic to treat persistent wounds, although the precise platelet-derived growth factors responsible for these beneficial effects have not been fully elucidated. The aim of thisstudy was to investigate the specific role of platelet-derived TGF1in cutaneous wound healing.Using a transgenic mouse with a targeted deletion of TGF1in megakaryocytes and platelets (TGFβ1fl/fl.PF4-Cre), we show for the first-time platelet-derived TGF1 contributes to epidermal and dermal thickening and cellular turnover after excisional skin wounding. In vitrostudies demonstrate human dermal fibroblasts stimulated with platelet lysate containing high levels of platelet-derived TGF1 did not exhibit enhanced collagen deposition or proliferation, suggestingthat platelet-derived TGF1 is not a key promoter of these wound healing processes.Interestingly, human keratinocytes displayed enhanced TGF1-driven proliferation in response to platelet lysate, reminiscent of our in vivofindings. In summary, our novel findings define andemphasiseanimportant roleof platelet-derived TGF1in epidermal remodellingand regenerationprocessesduring cutaneous wound healing.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Chong, Deborah L.H
Labelle, Myriam
Rodriguez-Justo, Manuel
Hughes, Sian
Holmes, Alan M.
Scotton, Chris J.
Porter, Joanna C.
Date : 18 February 2020
Funders : Department of Health’s NIHR Biomedical Research Centre, Medical Research Council (MRC)
OA Location :
Grant Title : Medical Research Council (MRC)
Copyright Disclaimer : This article is protected by copyright. All rights reserved.
Uncontrolled Keywords : Blood platelets; Epidermis; Keratinocytes; Platelet-rich plasma; Skin;Transforming Growth Factor Beta; Transgenic mouse; Wound healing
Depositing User : James Marshall
Date Deposited : 20 Feb 2020 14:33
Last Modified : 15 Feb 2021 02:08

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