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Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments

Glaize, Ayanna, Gutierrez-Rodriguez, Eduardo, Hanning, Irene, Diaz-Sanchez, Sandra, Gunter, Chris, van Vliet, Arnoud, Watson, Wes and Thakur, Siddhartha (2019) Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments International Journal of Food Microbiology, 319, 108472.

Glaize_IJFM_finalversion.pdf - Accepted version Manuscript

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The interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design, close to fresh produce, and growers frequently move between the two production environments. The primary objectives of this study were to 1) determine the transmission of STEC or enteric pathogens from small and large animal herds or operations to fresh produce on sustainable farms in TN and NC, 2) identify the possible sources that impact transmission of AMR E. coli, specifically STEC on these systems, and 3) WGS to characterize recovered E. coli from these sources. Samples were collected from raw and composted manure, environment, and produce sources. The serotype, virulence, and genotypic resistance profile were determined using the assembled genome sequences sequenced by Illumina technology. Broth microdilution was used to determine the antimicrobial susceptibility of each isolate against a panel of fourteen antimicrobials. The prevalence of E. coli increased during the summer season for all sources tested. ParSNP trees generated demonstrated that the transmission of AMR E. coli is occurring between animal feeding operations and fresh produce. Ten isolates were identified as serotype O45, a serotype that is associated with the “Big Six” group that is frequently linked with foodborne outbreaks caused by non-O157 E. coli. However, these isolates did not possess the stx gene. The highest frequency of resistance was detected against streptomycin (n=225), ampicillin (n=190) and sulfisoxazole FIS (n=140). A total of 35 (13.7%) isolates from two TN farms were positive for the blaCMY (n=5) and blaTEM (n=32) genes. The results of this study show the potential of AMR E. coli transmission between animal feeding operations and fresh produce, and more studies are recommended to study this interaction and prevent dissemination in sustainable farming systems.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
Glaize, Ayanna
Gutierrez-Rodriguez, Eduardo
Hanning, Irene
Diaz-Sanchez, Sandra
Gunter, Chris
van Vliet,
Watson, Wes
Thakur, Siddhartha
Date : 30 December 2019
Copyright Disclaimer : © 2019 Published by Elsevier B.V.T
Uncontrolled Keywords : Pathogen transmission, Sustainable production systems, Antimicrobial resistance, Phylotyping, non-O157 E. coli
Depositing User : James Marshall
Date Deposited : 04 Feb 2020 11:06
Last Modified : 04 Feb 2020 11:06

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