Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis

Dalley, Deanna, Lesellier, Sandrine, Salguero, Francisco J. and Chambers, Mark A. (2019) Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis Veterinary Sciences, 6 (4). pp. 89-99.

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Abstract

European badgers are a wildlife reservoir of bovine tuberculosis in parts of Great Britain. Accurate diagnosis of tuberculosis in badgers is important for the development of strategies for the control of the disease. Sensitive serological tests for badger TB are needed for reasons such as cost and simplicity. Assay of mucosal IgA could be useful for diagnosing respiratory pathogens such as Mycobacterium bovis and for monitoring the response to mucosal vaccination. To develop an IgA assay, we purified secretory IgA from badger bile, identifying secretory component (SC), heavy chain (HC) and light chain (LC), at 66, 46 and 27 Kda, respectively, on the basis of size comparison with other species. Monoclonal antibodies (mAbs) were generated to purified IgA.We selected two for ELISA development. The detection limit of the IgA-specific mAbs was found to be approximately 20 ng/mL when titrated against purified badger bile. One monoclonal antibody specific for badger IgA was used to detect IgA in serum and tracheal aspirate with specificity to an immunodominant antigen of M. bovis. An M. bovis infection dose-dependent IgA response was observed in experimentally infected badgers. IgA was also detected by immunohistochemistry in the lungs of bTB-infected badgers. With further characterisation, these represent new reagents for the study of the IgA response in badgers.

Item Type:	Article
Divisions :	Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :	Name Email ORCID Dalley, Deanna Lesellier, Sandrine Salguero, Francisco J. Chambers, Mark A. m.chambers@surrey.ac.uk
Date :	2 November 2019
Funders :	Department for Environment, Food and Rural Affairs
DOI :	10.3390/vetsci6040089
Copyright Disclaimer :	© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords :	Immunoglobulin A; Badger; Tuberculosis
Depositing User :	Diane Maxfield
Date Deposited :	13 Nov 2019 13:04
Last Modified :	13 Nov 2019 13:04
URI:	http://epubs.surrey.ac.uk/id/eprint/853124

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