Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach

Henslee, Erin A., Torcal Serrano, Ruth M., Labeed, Fatima H., Jabr, Rita I., Fry, Christopher H., Hughes, Michael P. and Hoettges, Kai F. (2016) Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach The Analyst, 141 (23). pp. 6408-6415.

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Abstract

A loss of ability of cells to undergo apoptosis (programmed cell death, whereby the cell ceases to function and destroys itself) is commonly associated with cancer, and many anti-cancer interventions aim to restart the process. Consequently, the accurate quantification of apoptosis is essential in understanding the function and performance of new anti-cancer drugs. Dielectrophoresis has previously been demonstrated to detect apoptosis more rapidly than other methods, and is low-cost, label-free and rapid, but has previously been unable to accurately quantify cells through the apoptotic process because cells in late apoptosis disintegrate, making cell tracking impossible. In this paper we use a novel method based on light absorbance and multi-population tracking to quantify the progress of apoptosis, benchmarking against conventional assays including MTT, trypan blue and Annexin-V. Analyses are performed on suspension and adherent cells, and using two apoptosis-inducing agents. IC₅₀ measurements compared favourably to MTT and were superior to trypan blue, whilst also detecting apoptotic progression faster than Annexin-V.

Item Type:	Article
Divisions :	Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :	Name Email ORCID Henslee, Erin A. e.henslee@surrey.ac.uk Torcal Serrano, Ruth M. Labeed, Fatima H. F.Labeed@surrey.ac.uk Jabr, Rita I. rita.jabr@surrey.ac.uk Fry, Christopher H. C.H.Fry@surrey.ac.uk Hughes, Michael P. M.Hughes@surrey.ac.uk Hoettges, Kai F. K.Hoettges@surrey.ac.uk
Date :	19 October 2016
Funders :	South East of England Development Agency, Finance South East, Labtech International
DOI :	10.1039/C6AN01596D
Copyright Disclaimer :	© The Royal Society of Chemistry 2016. This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence.
Depositing User :	Clive Harris
Date Deposited :	30 Sep 2019 10:02
Last Modified :	30 Sep 2019 10:02
URI:	http://epubs.surrey.ac.uk/id/eprint/852834

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