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Antigenic relationship among zoonotic flaviviruses from Italy

Lorusso, Alessio, Marini, Valeria, Di Gennaro, Annapia, Ronchi, Gaetano Federico, Casaccia, Claudia, Carelli, Grazia, Passantino, Giuseppe, D’Alterio, Nicola, D’Innocenzo, Vincenzo, Savini, Giovanni , Monaco, Federica and Horton, Daniel (2019) Antigenic relationship among zoonotic flaviviruses from Italy Infection, Genetics and Evolution, 68. pp. 91-97.

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Here we report studies of the antigenic relationship of West Nile virus (WNV) and Usutu virus (USUV), two zoonotic flaviviruses from Italy, together with a Japanese encephalitis virus (JEV) strain and compared them with their genetic relationship using the immunodominant viral E protein. Thirty-nine isolates and reference strains were inactivated and used to immunize rabbits to produce hyper immune sera. Serum samples were tested by neutralization against all isolates and results visualized by generating antigenic map. Strains of WNV, USUV, and JEV grouped in separate clusters on the antigenic map. JEV was closer antigenically to USUV (mean of 3.5 Antigenic Unit, AU, equivalent to a 2-fold change in antibody titer) than to WNV strains (mean of 6 AU). A linear regression model predicted, on average, one unit of antigenic change, equivalent to a 2-fold change in antibody titer, for every 22 amino acid substitutions in the E protein ectodomain. Overall, antigenic map was demonstrated to be robust and consistent with phylogeny of the E protein. Indeed, the map provided a reliable means of visualizing and quantifying the relationship between these flaviviruses. Further antigenic analyses employing representative strains of extant serocomplexes are currently underway. This will provide a more in deep knowledge of antigenic relationships between flaviviruses.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
Lorusso, Alessio
Marini, Valeria
Di Gennaro, Annapia
Ronchi, Gaetano Federico
Casaccia, Claudia
Carelli, Grazia
Passantino, Giuseppe
D’Alterio, Nicola
D’Innocenzo, Vincenzo
Savini, Giovanni
Monaco, Federica
Date : March 2019
DOI : 10.1016/j.meegid.2018.11.023
Copyright Disclaimer : © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Uncontrolled Keywords : Flaviviruses; West Nile; Usutu; Antigenic cartography; Antigenic characterization
Depositing User : Melanie Hughes
Date Deposited : 30 Nov 2018 16:27
Last Modified : 03 Dec 2019 02:08

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