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mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis

Choe, Junho, Lin, Shuibin, Zhang, Wencai, Liu, Qi, Wang, Longfei, Ramirez-Moya, Julia, Du, Peng, Kim, Wantae, Tang, Shaojun, Sliz, Piotr , Santisteban, Pilar, George, Rani E, Richards, William G, Wong, Kwok-Kin, Locker, Nicolas, Slack, Frank J and Gregory, Richard I (2018) mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis Nature, 561. pp. 556-560.

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N6-Methyladenosine (m6A), the most abundant posttranscriptional messenger RNA (mRNA) modification, is emerging as an important regulator of gene expression1. Manipulation of m6A impacts different developmental and biological processes, and altered m6A homeostasis is linked to cancer2-5. m6A is catalyzed by METTL3 and enriched in the 3’ untranslated region (3’ UTR) of a large subset of mRNAs at sites close to the stop codon1. METTL3 can promote translation but the mechanism and widespread relevance remain unknown2. Here we show that METTL3 enhances translation only when tethered to reporter mRNA at sites close to the stop codon supporting a mRNA looping mechanism for ribosome recycling and translational control. Electron microscopy reveals the topology of individual polyribosomes with single METTL3 foci found in close proximity to 5’ cap-binding proteins. We identify a direct physical and functional interaction between METTL3 and the eukaryotic translation initiation factor 3 subunit h (eIF3h). METTL3 promotes translation of a large subset of oncogenic mRNAs, including Bromodomain-containing protein 4 (BRD4) that are also m6A-modified in human primary lung tumors. The METTL3-eIF3h interaction is required for enhanced translation, formation of densely packed polyribosomes, and oncogenic transformation. METTL3 depletion inhibits tumorigenicity and sensitizes lung cancer cells to BRD4 inhibition. These findings uncover a mRNA looping mechanism of translation control and identify METTL3-eIF3h as a potential cancer therapeutic target.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Choe, Junho
Lin, Shuibin
Zhang, Wencai
Liu, Qi
Wang, Longfei
Ramirez-Moya, Julia
Du, Peng
Kim, Wantae
Tang, Shaojun
Sliz, Piotr
Santisteban, Pilar
George, Rani E
Richards, William G
Wong, Kwok-Kin
Slack, Frank J
Gregory, Richard I
Date : 19 September 2018
DOI : 10.1038/s41586-018-0538-8
Copyright Disclaimer : Copyright 2018 The Author(s). Published by Nature Publishing Group
Uncontrolled Keywords : N6-methyladenosine; m6A; METTL3; polysome; translation; closed loop; eIF3h; lung adenocarcinoma; oncogene; BRD4; JQ1
Depositing User : Melanie Hughes
Date Deposited : 21 Aug 2018 15:24
Last Modified : 20 Mar 2019 02:08

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