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Influence of age on the absorption, metabolism, and excretion of cocoa flavanols in healthy subjects

Rodriguez-Mateos, Ana, Cifuentes-Gomez, Tania, Gonzalez-Salvador, Isidro, Ottaviani, Javier I., Schroeter, Hagen, Kelm, Malte, Heiss, Christian and Spencer, Jeremy P. E. (2015) Influence of age on the absorption, metabolism, and excretion of cocoa flavanols in healthy subjects Molecular Nutrition & Food Research, 59 (8). pp. 1504-1512.

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An understanding of the pharmacokinetics of structurally related (−)‐epicatechin metabolites (SREM) is a prerequisite for considering cocoa flavanols (CF) in the context of dietary recommendations. The objective of this study was to compare the absorption, metabolism, and excretion of SREM in healthy young and elderly Caucasian men.

Methods and results

Intraindividual variability of SREM was assessed in seven young subjects, after consuming 10.7 mg CF/kg body weight (BW) on two occasions separated by 1 week. The effect of age on flavanols ADME was assessed in 20 young (18–35 years) and 20 elderly (65–80 years) healthy male subjects receiving 5.3 and 10.7 mg total CF/kg BW or 1 g of acetaminophen as a control to compare differences in Phase II metabolism on three days separated by 1 week of wash‐out. Blood and urine samples were collected for 24‐h post consumption. The intraindividual variation, measured as CV(%) with respect to the area‐under‐the‐curve of the concentration over time (AUC(0‐6h)) of SREM, was 16%, while the interindividual variation in AUC(0‐6h), was 38%, comparable to acetaminophen (39%). The AUC(0‐6h) and the 24‐h excretion of total SREM was not significantly different between young and elderly subjects. At the high intake amount, the AUC(0‐6h) of (−)‐epicatechin‐3′‐β‐D‐glucuronide was greater in elderly subjects, whereas the AUC(0‐6h) of 3′‐O‐methyl‐(−)‐epicatechin‐5‐sulfate and 3′‐O‐methyl‐(−)‐epicatechin‐7‐sulfate as well as the 24‐h urinary excretion of γ‐valerolactone metabolites were lower in the elderly.


Cocoa flavanols are absorbed, metabolized, and excreted in healthy young and elderly subjects with relatively small differences between the two groups.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Rodriguez-Mateos, Ana
Cifuentes-Gomez, Tania
Gonzalez-Salvador, Isidro
Ottaviani, Javier I.
Schroeter, Hagen
Kelm, Malte
Spencer, Jeremy P. E.
Date : 18 May 2015
DOI : 10.1002/mnfr.201500091
Depositing User : Melanie Hughes
Date Deposited : 16 Aug 2018 09:17
Last Modified : 16 Aug 2018 09:17

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