High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions
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Phillips, Jack O, Butt, Louise E, Henderson, Charlotte A, Devonshire, Martin, Healy, Jess, Conway, Stuart J, Locker, Nicolas, Pickford, Andrew R, Vincent, Helen A and Callaghan, Anastasia J (2018) High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions Nucleic Acids Research, 46 (14).
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Official URL: https://doi.org/10.1093/nar/gky410
Abstract
We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilized, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA–small molecule and RNA–RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greater user-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterization.
| Item Type: | Article | |||||||||||||||||||||||||||||||||
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| Divisions : | Faculty of Health and Medical Sciences > School of Biosciences and Medicine | |||||||||||||||||||||||||||||||||
| Authors : |
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| Date : | 28 May 2018 | |||||||||||||||||||||||||||||||||
| Funders : | Biotechnology and Biological Sciences Research Council (BBSRC) | |||||||||||||||||||||||||||||||||
| DOI : | 10.1093/nar/gky410 | |||||||||||||||||||||||||||||||||
| Copyright Disclaimer : | © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Supplementary data | |||||||||||||||||||||||||||||||||
| Uncontrolled Keywords : | Protein-nucleic acid interaction; RNA characterisation and manipulation | |||||||||||||||||||||||||||||||||
| Depositing User : | Clive Harris | |||||||||||||||||||||||||||||||||
| Date Deposited : | 07 Jun 2018 12:10 | |||||||||||||||||||||||||||||||||
| Last Modified : | 11 Dec 2018 11:24 | |||||||||||||||||||||||||||||||||
| URI: | http://epubs.surrey.ac.uk/id/eprint/847011 |
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