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Imaging deep skeletal muscle structure using a high-sensitivity ultrathin side-viewing optical coherence tomography needle probe

Yang, X., Lorenser, D., McLaughlin, R.A., Kirk, R.W., Edmond, M., Simpson Cather, M., Grounds, M.D. and Sampson, David (2014) Imaging deep skeletal muscle structure using a high-sensitivity ultrathin side-viewing optical coherence tomography needle probe Biomedical Optics Express, 5 (1). pp. 136-148.

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We have developed an extremely miniaturized optical coherence tomography (OCT) needle probe (outer diameter 310 μm) with high sensitivity (108 dB) to enable minimally invasive imaging of cellular structure deep within skeletal muscle. Three-dimensional volumetric images were acquired from ex vivo mouse tissue, examining both healthy and pathological dystrophic muscle. Individual myofibers were visualized as striations in the images. Degradation of cellular structure in necrotic regions was seen as a loss of these striations. Tendon and connective tissue were also visualized. The observed structures were validated against coregistered hematoxylin and eosin (H&E) histology sections. These images of internal cellular structure of skeletal muscle acquired with an OCT needle probe demonstrate the potential of this technique to visualize structure at the microscopic level deep in biological tissue in situ.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
Yang, X.
Lorenser, D.
McLaughlin, R.A.
Kirk, R.W.
Edmond, M.
Simpson Cather, M.
Grounds, M.D.
Date : 2014
DOI : 10.1364/BOE.5.000136
Uncontrolled Keywords : Biological tissues, Cellular structure, Connective tissues, High-sensitivity, Microscopic levels, Minimally invasive imaging, Outer diameters, Volumetric images, Collagen, Muscle, Needles, Probes, Optical tomography, animal tissue, article, astigmatism, cell structure, cone beam computed tomography, connective tissue, interferometer, lens, machine, mouse, muscle fibril, muscle necrosis, nonhuman, optic nerve fiber, optical coherence tomography device, optical coherence tomography needle probe, quantitative analysis, refraction index, scanning electron microscope, sensitivity analysis, signal noise ratio, skeletal muscle, spectral sensitivity
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 05 Jun 2018 09:26
Last Modified : 19 Sep 2018 11:32

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