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Immunoglobulin gene analysis as a tool for investigating human immune responses

Dunn-Walters, Deborah, Townsend, C, Sinclair, Emma and Stewart, Alexander (2018) Immunoglobulin gene analysis as a tool for investigating human immune responses Immunological Reviews, 284 (1). pp. 132-147.

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The human immunoglobulin repertoire is a hugely diverse set of sequences that are formed by processes of gene rearrangement, heavy and light chain gene assortment, class switching and somatic hypermutation. Early B cell development produces diverse IgM and IgD B cell receptors on the B cell surface, resulting in a repertoire that can bind many foreign antigens but which has had self-reactive B cells removed. Later antigen-dependent development processes adjust the antigen affinity of the receptor by somatic hypermutation. The effector mechanism of the antibody is also adjusted, by switching the class of the antibody from IgM to one of seven other classes depending on the required function. There are many instances in human biology where positive and negative selection forces can act to shape the immunoglobulin repertoire and therefore repertoire analysis can provide useful information on infection control, vaccination efficacy, autoimmune diseases and cancer. It can also be used to identify antigen-specific sequences that may be of use in therapeutics. The juxtaposition of lymphocyte development and numerical evaluation of immune repertoires has resulted in the growth of a new sub-speciality in immunology where immunologists and computer scientists/physicists collaborate to assess immune repertoires and develop models of immune action.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Townsend, C
Date : 26 June 2018
Funders : BBSRC; MRC
DOI : 10.1111/imr.12659
Copyright Disclaimer : © 2018 The Authors. Immunological Reviews Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : B cell Antibody repertoire human
Additional Information : This article is part of a series of reviews covering Characterization of the Immunologic Repertoire appearing in Volume 284 of Immunological Reviews.
Depositing User : Melanie Hughes
Date Deposited : 13 Apr 2018 10:02
Last Modified : 11 Dec 2018 11:24

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