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Proteomics Technique Opens New Frontiers in Mobilome Research

Davidson, Andrew D., Matthews, David A. and Maringer, Kevin (2017) Proteomics Technique Opens New Frontiers in Mobilome Research Mobile Genetic Elements, 7 (4), e1362494. pp. 1-9.

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A large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. The sum of these elements is called the ‘mobilome’, which in eukaryotes is made up mostly of transposons. Transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the ‘domestication’ of transposon proteins for cellular functions. Although ‘omics studies of mobilome genomes and transcriptomes are common, technical challenges have hampered high-throughput global proteomics analyses of transposons. In a recent paper, we overcame these technical hurdles using a technique called ‘proteomics informed by transcriptomics' (PIT), and thus published the first unbiased global mobilome-derived proteome for any organism (using cell lines derived from the mosquito Aedes aegypti). In this commentary, we describe our methods in more detail, and summarise our major findings. We also use new genome sequencing data to show that, in many cases, the specific genomic element expressing a given protein can be identified using PIT. This proteomic technique therefore represents an important technological advance that will open new avenues of research into the role that proteins derived from transposons and other repetitive and sequence diverse genetic elements, such as endogenous retroviruses, play in health and disease.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Davidson, Andrew D.
Matthews, David A.
Date : 1 August 2017
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.1080/2159256X.2017.1362494
Copyright Disclaimer : © 2017 Andrew D. Davidson, David A. Matthews, and Kevin Maringer. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License ( 4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
Uncontrolled Keywords : Mobilome; Transposon proteomics; LTR retrotransposon; Endogenous retrovirus; Repetitive element; Proteomics informed by transcriptomics (PIT); Aedes aegypti
Depositing User : Clive Harris
Date Deposited : 15 Aug 2017 08:18
Last Modified : 11 Dec 2018 11:23

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