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Phospholipid oxidation and carotenoid supplementation in Alzheimer’s disease patients

Ademowo, OS, Dias, HKI, Milic, I, Devitt, A, Moran, R, Mulcahy, R, Howard, AN, Nolan, JM and Griffiths, HR (2017) Phospholipid oxidation and carotenoid supplementation in Alzheimer’s disease patients Free Radical Biology and Medicine, 108. pp. 77-85.

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Alzheimer's disease (AD) is a progressive, neurodegenerative disease, characterised by decline of memory, cognitive function and changes in behaviour. Generic markers of lipid peroxidation are increased in AD and reactive oxygen species have been suggested to be involved in the aetiology of cognitive decline. Carotenoids are depleted in AD serum, therefore we have compared serum lipid oxidation between AD and age-matched control subjects before and after carotenoid supplementation. The novel oxidised phospholipid biomarker 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) was analysed using electrospray ionisation tandem mass spectrometry (MS) with multiple reaction monitoring (MRM), 8-isoprostane (IsoP) was measured by ELISA and ferric reducing antioxidant potential (FRAP) was measured by a colorimetric assay. AD patients (n=21) and healthy age-matched control subjects (n=16) were supplemented with either Macushield™ (10 mg meso-zeaxanthin, 10 mg lutein, 2 mg zeaxanthin) or placebo (sunflower oil) for six months. The MRM-MS method determined serum POVPC sensitively (from 10 µl serum) and reproducibly (CV=7.9%). At baseline, AD subjects had higher serum POVPC compared to age-matched controls, (p=0.017) and cognitive function was correlated inversely with POVPC (r=−0.37; p=0.04). After six months of carotenoid intervention, serum POVPC was not different in AD patients compared to healthy controls. However, POVPC was significantly higher in control subjects after six months of carotenoid intervention compared to their baseline (p=0.03). Serum IsoP concentration was unrelated to disease or supplementation. Serum FRAP was significantly lower in AD than healthy controls but was unchanged by carotenoid intervention (p=0.003). In conclusion, serum POVPC is higher in AD patients compared to control subjects, is not reduced by carotenoid supplementation and correlates with cognitive function

Item Type: Article
Subjects : Biosciences
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Ademowo, OS
Dias, HKI
Milic, I
Devitt, A
Moran, R
Mulcahy, R
Howard, AN
Nolan, JM
Date : 14 March 2017
DOI : 10.1016/j.freeradbiomed.2017.03.008
Copyright Disclaimer : © 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (
Uncontrolled Keywords : Oxidative stress Lipid peroxidation POVPC Mass spectrometry Lutein Meso-zeaxanthin Zeaxanthin Supplementation Cognitive function
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:48
Last Modified : 16 Jan 2019 18:06

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