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Functional analysis of lymphostatin homologues in enterohaemorrhagic Escherichia coli.

Abu-Median, AB, van Diemen, PM, Dziva, F, Vlisidou, I, Wallis, TS and Stevens, MP (2006) Functional analysis of lymphostatin homologues in enterohaemorrhagic Escherichia coli. FEMS Microbiol Lett, 258 (1). pp. 43-49.

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Enteropathogenic Escherichia coli contain a large chromosomal gene (lifA) that encodes lymphostatin, a predicted 365 kDa protein that inhibits the mitogen-activated proliferation of peripheral blood lymphocytes and lamina propria mononuclear cells and the synthesis of proinflammatory cytokines. Non-O157 serotypes of enterohaemorrhagic E. coli (EHEC) contain a highly homologous gene, designated efa1 (EHEC factor for adherence), which influences adherence to epithelial cells in vitro and intestinal colonization in calves. Serotype O157:H7 EHEC strains contain a truncated version of this gene (efa1') and a pO157-encoded homologue of lifA/efa1 (toxB). Here we report for the first time that efa1 inhibits mitogen-activated proliferation of bovine peripheral blood lymphocytes by EHEC O103:H2, but that E. coli K-12 strains expressing the N-terminal and central portions of the protein lack activity. While a Shiga toxin-negative E. coli O157:H7 strain was shown to possess lymphostatin-like activity, deletion of efa1' or toxB, singly or in combination, failed to significantly relieve the inhibitory effect.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Abu-Median, AB
van Diemen, PM
Vlisidou, I
Wallis, TS
Stevens, MP
Date : May 2006
DOI : 10.1111/j.1574-6968.2006.00198.x
Uncontrolled Keywords : Animals, Bacterial Toxins, Cattle, Escherichia coli O157, Escherichia coli Proteins, Lymphocyte Activation, Protein Structure, Tertiary
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:39
Last Modified : 24 Jan 2020 19:46

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