Recombinant ovine interferon gamma inhibits the multiplication of Chlamydia psittaci in ovine cells.

Graham, SP, Jones, GE, MacLean, M, Livingstone, M and Entrican, G (1995) Recombinant ovine interferon gamma inhibits the multiplication of Chlamydia psittaci in ovine cells. J Comp Pathol, 112 (2). pp. 185-195.

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Abstract

The local production of interferon-gamma (IFN-gamma) in sheep in response to Chlamydia psittaci was measured by cannulation of the efferent lymph duct draining the site of challenge inoculation. Peak production of IFN-gamma (256 U/ml) was detected 24 h after challenge. Based on these physiological data, functional studies were carried out in vitro to determine the effect of recombinant ovine (rOv) IFN-gamma on the multiplication of C. psittaci in ovine fibroblasts. IFN-gamma inhibited the multiplication of C. psittaci in ovine cells over a range of concentrations (250 U/ml to 2.5 U/ml) in a dose-dependent manner. The inhibition of chlamydial multiplication was most pronounced when cells were treated with rOvIFN-gamma for 24 h before infection. The addition of exogenous L-tryptophan (500 micrograms/ml) to cultures within 48th of infection abrogated the anti-chlamydial effect of rOvIFN-gamma thus suggesting that tryptophan deprivation is an anti-chlamydial mechanism induced by rOvIFN-gamma in these ovine cells.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Name Email ORCID Graham, SP s.graham@surrey.ac.uk Jones, GE MacLean, M Livingstone, M Entrican, G
Date :	February 1995
Uncontrolled Keywords :	Animals, Chlamydophila psittaci, Dose-Response Relationship, Drug, Female, Fibroblasts, Interferon-gamma, Lymph, Psittacosis, Recombinant Proteins, Sheep, Sheep Diseases, Time Factors, Tryptophan
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:22
Last Modified :	24 Jan 2020 19:08
URI:	http://epubs.surrey.ac.uk/id/eprint/827520

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