University of Surrey

Test tubes in the lab Research in the ATI Dance Research

In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization.

Holland, PW, Harper, SJ, McVey, JH and Hogan, BL (1987) In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. J Cell Biol, 105 (1). pp. 473-482.

Full text not available from this repository.


In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. High levels of SPARC RNA are found in osteoblasts and odontoblasts. In addition, high grain counts are associated with a variety of other cell types in the embryo and newborn mouse, including parietal endoderm, deciduum, whisker follicles (connective tissue sheath), peripheral nerve trunk, skin (dermis), and stomach (submucosa). Spatially restricted but high levels of SPARC mRNA are also seen in the adult adrenal glands, testis, and ovary. This pattern of differential gene expression demands a reassessment of the function originally proposed for osteonectin, and predicts a much wider role for the protein in a variety of biological processes.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Holland, PW
Harper, SJ
Hogan, BL
Date : July 1987
Uncontrolled Keywords : Adrenal Glands, Age Factors, Animals, Animals, Newborn, Carrier Proteins, Female, Gene Expression Regulation, Male, Mice, Mice, Inbred CBA, Nucleic Acid Hybridization, Organ Specificity, Osteonectin, Ovary, RNA, RNA, Complementary, RNA, Messenger, Testis
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:16
Last Modified : 24 Jan 2020 18:52

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800