A cDNA clone encoding the mouse Qa-1a histocompatibility antigen and proposed structure of the putative peptide binding site.

Connolly, DJ, Cotterill, LA, Hederer, RA, Thorpe, CJ, Travers, PJ, McVey, JH, Dyson, J and Robinson, PJ (1993) A cDNA clone encoding the mouse Qa-1a histocompatibility antigen and proposed structure of the putative peptide binding site. J Immunol, 151 (11). pp. 6089-6098.

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Abstract

We have isolated a cDNA clone which encodes the Qa-1a histocompatibility Ag from a library prepared from Con A-activated B10.BR mouse spleen cells. The clone encodes a protein of 322 amino acids with three potential N-glycosylation sites. The coding sequence shows strongest similarity with that of the T23d gene of DBA/2 mice which encodes the Qa-1b molecule. Molecular modeling of the putative peptide-combining site indicates most of the differences between Qa-1a and Qa-1b are located peripheral to the binding cleft, with only two amino acid substitutions, at positions 9 and 24, which might affect peptide binding. Many features of the Qa-1 binding cleft are also conserved in the rat RTBM.1 and in human HLA-E molecules. This suggests that all of these molecules may associate with structurally similar peptides.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Name Email ORCID Connolly, DJ Cotterill, LA Hederer, RA Thorpe, CJ Travers, PJ McVey, JH j.mcvey@surrey.ac.uk Dyson, J Robinson, PJ
Date :	1 December 1993
Uncontrolled Keywords :	Amino Acid Sequence, Animals, Base Sequence, Binding Sites, DNA, Complementary, H-2 Antigens, Histocompatibility Antigens Class I, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Protein Biosynthesis, Rabbits
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:16
Last Modified :	24 Jan 2020 18:51
URI:	http://epubs.surrey.ac.uk/id/eprint/827095

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