Comparison of three monoclonal antibodies for use in immunohistochemical detection of bovine spongiform encephalopathy protease-resistant prion protein.
Salguero, FJ, Díaz-San, SF, Brun, A, Cano, MJ and Torres, JM (2006) Comparison of three monoclonal antibodies for use in immunohistochemical detection of bovine spongiform encephalopathy protease-resistant prion protein. J Vet Diagn Invest, 18 (1). pp. 106-109.
Full text not available from this repository.Abstract
Confirmatory diagnosis of prion diseases in humans and animals relies on the histopathological examination and immunodetection of the protease-resistant isoform of prion protein (PrPres). The generation of novel PrP-specific monoclonal antibodies (MAbs) has greatly improved diagnostic methodology and basic research on prion diseases as well. In this study, the performance of 3 different PrP-specific MAbs in recognizing brain PrPres deposits from cows affected with bovine spongiform encephalopathy (BSE) was compared by using a standard immunohistochemical technique under different pretreatment conditions. All antibodies showed similar reactivity after denaturing treatment. However, greater differences were found among them after proteinase K treatment, even in the absence of a denaturing step. In fact, 1 MAb (2A11) was able to react with PrPres deposits in the absence of a denaturing step, yielding the strongest signal and confirming the usefulness of MAb 2A11 in immunohistochemistry for the diagnosis of BSE.
Item Type: | Article |
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Divisions : | Surrey research (other units) |
Authors : | Salguero, FJ, Díaz-San, SF, Brun, A, Cano, MJ and Torres, JM |
Date : | January 2006 |
Uncontrolled Keywords : | Amino Acid Sequence, Animals, Antibodies, Monoclonal, Brain Stem, Cattle, Encephalopathy, Bovine Spongiform, Endopeptidase K, Epitopes, Immunohistochemistry, Molecular Sequence Data, Prions, Protein Denaturation |
Related URLs : | |
Depositing User : | Symplectic Elements |
Date Deposited : | 17 May 2017 10:08 |
Last Modified : | 24 Jan 2020 18:33 |
URI: | http://epubs.surrey.ac.uk/id/eprint/826554 |
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