University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Histological measurement of fat content of liver of dairy cows.

Collins, RA, Reid, IM, Williams, LA and Dennis, PF (1985) Histological measurement of fat content of liver of dairy cows. Journal of Comparative Pathology, 95 (3). pp. 437-441.

Full text not available from this repository.


A total of 275 liver biopsies were performed on dairy cows in 6 Friesian and one Guernsey herd during the first 2 weeks after calving. Liver samples were processed by 2 independent histological procedures: (a) formalin-fixed frozen sections were stained with oil-red O (ORO), or (b) samples fixed in glutaraldehyde-osmium tetroxide were embedded in plastic and sections stained with toluidine blue (TOLB). The sections were then subjected to stereological point-counting procedures to assess the quantities of stainable fat present within the liver cells. Estimates of liver cell fat by the 2 techniques were highly correlated, although those obtained by the TOLB method were consistently lower than those obtained by the ORO method. The analytical sampling error was slightly lower in the TOLB method. The simpler ORO method should prove an acceptable alternative in the routine histology laboratory to the tedious and technically demanding TOLB method.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Psychology
Authors :
Collins, RA
Reid, IM
Dennis, PF
Date : July 1985
Uncontrolled Keywords : Adipose Tissue, Animals, Biopsy, Needle, Cattle, Female, Lipids, Liver
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:56
Last Modified : 29 Oct 2018 10:11

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800