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Rapid quantification of quinine and its major metabolite (3S)-3-hydroxyquinine in diluted urine by UPLC-MS/MS.

Heaton, J, Rahmioglu, N, Ahmadi, KR, Legido-Quigley, C and Smith, NW (2011) Rapid quantification of quinine and its major metabolite (3S)-3-hydroxyquinine in diluted urine by UPLC-MS/MS. J Pharm Biomed Anal, 55 (3). pp. 494-499.

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A rapid UPLC-MS/MS quantitative assay for the quantification of quinine and (3S)-3-hydroxyquinine requiring minimal sample pre-treatment - dilute-and-shoot type approach - has been developed. The assay was run at 0.6mL/min using gradient elution with (pH 10; 10mM) ammonium bicarbonate and methanol with a total cycle time of 2.5min on a 50mm×2.1mm ID, 1.7μm Acquity BEH column. Peak shapes were highly symmetrical allowing for accurate peak integration. Calibration curves for both analytes were constructed from 1.00 to 20.00ng/mL, yielding R(2) values >0.995. Intra- and inter-batch assay precision and accuracy were evaluated using 6 injections of QC solutions on 3 separate days (n=18) and were found to be within ±10% and 90-110% respectively. The method was shown to be suitable for quantitatively determining the ratio of quinine to (3S)-3-hydroxyquinine for a cohort of samples from an epidemiological study.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Heaton, J
Rahmioglu, N
Legido-Quigley, C
Smith, NW
Date : 1 June 2011
DOI : 10.1016/j.jpba.2011.02.004
Uncontrolled Keywords : Calibration, Chromatography, High Pressure Liquid, Humans, Limit of Detection, Quinidine, Quinine, Reference Standards, Reproducibility of Results, Solid Phase Extraction, Solutions, Tandem Mass Spectrometry
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:48
Last Modified : 24 Jan 2020 17:41

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