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Statins use a novel Nijmegen breakage syndrome-1-dependent pathway to accelerate DNA repair in vascular smooth muscle cells.

Mahmoudi, M, Gorenne, I, Mercer, J, Figg, N, Littlewood, T and Bennett, M (2008) Statins use a novel Nijmegen breakage syndrome-1-dependent pathway to accelerate DNA repair in vascular smooth muscle cells. Circ Res, 103 (7). pp. 717-725.

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Although the hydroxymethylglutaryl-coenzyme A reductase inhibitors (statins) are widely used in atherosclerosis to reduce serum cholesterol, statins have multiple other effects, including direct effects on cells of the vessel wall. Recently, DNA damage, including telomere shortening, has been identified in vascular smooth muscle cells (VSMCs) in human atherosclerosis. Although statins reduce DNA damage in vitro, the mechanisms by which they might protect DNA integrity in VSMCs are unknown. We show that human atherosclerotic plaque VSMCs exhibit increased levels of double-stranded DNA breaks and basal activation of DNA repair pathways involving ataxia telangiectasia-mutated (ATM) and the histone H2AX in vivo and in vitro. Oxidant stress induced DNA damage and activated DNA repair pathways in VSMCs. Statin treatment did not reduce oxidant stress or DNA damage but markedly accelerated DNA repair. Accelerated DNA repair required both the Nijmegen breakage syndrome (NBS)-1 protein and the human double minute protein Hdm2, accompanied by phosphorylation of Hdm2, dissociation of NBS-1 and Hdm2, inhibition of NBS-1 degradation, and accelerated phosphorylation of ATM. Statin treatment reduced VSMC senescence and telomere attrition in culture, accelerated DNA repair and reduced apoptosis in vivo after irradiation, and reduced ATM/ATR (ATM and Rad3-related) activity in atherosclerosis. We conclude that statins activate a novel mechanism of accelerating DNA repair, dependent on NBS-1 stabilization and Hdm2. Statin treatment may delay cell senescence and promote DNA repair in atherosclerosis.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Gorenne, I
Mercer, J
Figg, N
Littlewood, T
Bennett, M
Date : 26 September 2008
DOI : 10.1161/CIRCRESAHA.108.182899
Uncontrolled Keywords : Animals, Atherosclerosis, Cell Aging, Cell Cycle Proteins, Cells, Cultured, Cholesterol, DNA Breaks, Double-Stranded, DNA Repair, DNA-Binding Proteins, Endothelium, Vascular, Histones, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Mice, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Nuclear Proteins, Oxidative Stress, Phosphorylation, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-mdm2, Rabbits, Tumor Suppressor Proteins
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:46
Last Modified : 24 Jan 2020 17:37

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