University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Development of a method to measure preβHDL and αHDL apoA-I enrichment for stable isotopic studies of HDL kinetics

Li, X, Stolinski, M and Umpleby, AM (2012) Development of a method to measure preβHDL and αHDL apoA-I enrichment for stable isotopic studies of HDL kinetics

Full text not available from this repository.


Our understanding of HDL metabolism would be enhanced by the measurement of the kinetics of preβHDL, the nascent form of HDL, since elevated levels have been reported in patients with coronary artery disease. Stable isotope methodology is an established technique that has enabled the determination of the kinetics (production and catabolism) of total HDL apoA-I in vivo. The development of separation procedures to obtain a preβHDL fraction, the isotopic enrichment of which could then be measured, would enable further understanding of the pathways in vivo for determining the fate of preβHDL and the formation of αHDL. A method was developed and optimised to separate and measure preβHDL and αHDL apoA-I enrichment. Agarose gel electrophoresis was first used to separate lipoprotein subclasses, and then a 4-10 % discontinuous SDS-PAGE used to isolate apoA-I. Measures of preβHDL enrichment in six healthy subjects were undertaken following an infusion of L-[1-13C-leucine]. After isolation of preβ and αHDL, the isotopic enrichment of apoA-I for each fraction was measured by gas chromatography-mass spectrometry. PreβHDL apoA-I enrichment was measured with a CV of 0.51 % and aHDL apoA-I with a CV of 0.34 %. The fractional catabolic rate (FCR) of preβHDL apoA-I was significantly higher than the FCR of aHDL apoA-I (p < 0.005). This methodology can be used to selectively isolate preβ and aHDL apoA-I for the measurement of apoA-I isotopic enrichment for kinetics studies of HDL subclass metabolism in a research setting. © AOCS 2012.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Surrey research (other units)
Authors : Li, X, Stolinski, M and Umpleby, AM
Date : 1 October 2012
DOI : 10.1007/s11745-012-3703-0
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:46
Last Modified : 23 Jan 2020 15:46

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800