University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription.

Plant, N (2006) Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription. Methods Mol Biol, 320. pp. 343-354.

Full text not available from this repository.


Transcriptional activation of CYP gene expression by xenobiotics may have fundamental effects on body physiology. It may result in the altered pharmacokinetics of other chemicals in the body, both xenobiotic and endogenous substrates, potentially altering their effects. This may often result in no observable clinical effect, but in a significant number of cases these interactions lead to altered physiology or failure of a therapeutic drug. It is therefore important to be able to screen novel chemical entities for their ability to activate CYP gene expression. In addition, through mechanistic studies of how such transcriptional activation occurs, the ability to predict and avoid such potential interactions is improved. Reporter gene assays provide a simple, high-throughput methodology for examining the transcriptional activation of CYP gene expression by xenobiotics. They are suitable for use in screening as well as mechanistic studies and are of use in both the drug discovery/development and research arenas.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Date : 2006
DOI : 10.1385/1-59259-998-2:343
Uncontrolled Keywords : Cytochrome P-450 Enzyme System, Gene Expression Regulation, Enzymologic, Genes, Reporter, Humans, Transcription, Genetic, Tumor Cells, Cultured, Xenobiotics
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 08:50
Last Modified : 24 Jan 2020 15:11

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800