Differential cell composition and cytokine expression within lymph node granulomas from BCG vaccinated and non-vaccinated cattle experimentally infected with Mycobacterium bovis

Salguero Bodes, Francisco, Gibson, S, Garcia-Jimenez, W, Gough, J, Strickland, TS, Vordermeier, HM and Villarreal-Ramos, B (2016) Differential cell composition and cytokine expression within lymph node granulomas from BCG vaccinated and non-vaccinated cattle experimentally infected with Mycobacterium bovis Transboundary and Emerging Diseases, 64 (6). pp. 1734-1749.

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Abstract

Cattle vaccination against bovine tuberculosis (bTB) has been proposed as a supplementary method to help control the incidences of this disease. Bacillus Calmette-Guérin (BCG) is currently the only viable candidate vaccine for immunisation of cattle against bTB, caused by Mycobacterium bovis (M. bovis). In an attempt to characterise the differences in the immune response following M. bovis infection between BCG vaccinated and non-vaccinated animals, a combination of gross pathology, histopathology and immunohistochemical (IHC) analyses was used. BCG vaccination was found to significantly reduce the number of gross and microscopic lesions present within the lungs and lymph nodes. Additionally, the microscopically visible bacterial load of stage III and IV granulomas was reduced. IHC using cell surfaces markers revealed the number of CD68+ (macrophages), CD3+ (T-lymphocytes) and WC1+ cells (γδ T-cells) to be significantly reduced in lymph node granulomas of BCG vaccinated animals, when compared to non-vaccinated animals. B lymphocytes (CD79a+) were significantly increased in BCG-vaccinated cattle for granulomas at stages II, III and IV. IHC staining for iNOS showed a higher expression in granulomas from BCG vaccinated animals compared to non-vaccinated animals for all stages, being statistically significant in stages I and IV. TGFβ expression decreased alongside the granuloma development in non-vaccinated animals, whereas BCG vaccinated animals showed a slight increase alongside lesion progression. IHC analysis of the cytokines IFN-γ and TNFα demonstrated significantly increased expression within the lymph node granulomas of BCG vaccinated cattle. This is suggestive of a protective role for IFNγ and TNFα in the response to M. bovis infection. Findings shown in this study suggest that the use of BCG vaccine, can reduce the number and severity of lesions , induce a different phenotypic response and increase the local expression of key cytokines related to protection.

Item Type:	Article
Subjects :	Veterinary Medicine
Divisions :	Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :	Name Email ORCID Salguero Bodes, Francisco f.salguerobodes@surrey.ac.uk Gibson, S Garcia-Jimenez, W Gough, J Strickland, TS Vordermeier, HM Villarreal-Ramos, B
Date :	11 September 2016
DOI :	10.1111/tbed.12561
Copyright Disclaimer :	This is the peer reviewed version of the following article: Salguero, F. J., Gibson, S., Garcia-Jimenez, W., Gough, J., Strickland, T. S., Vordermeier, H. M. and Villarreal-Ramos, B. (2016), Differential Cell Composition and Cytokine Expression Within Lymph Node Granulomas from BCG-Vaccinated and Non-vaccinated Cattle Experimentally Infected with Mycobacterium bovis. Transbound Emerg Dis. doi:10.1111/tbed.12561, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/tbed.12561/full. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords :	Bovine tuberculosis, BCG, immunohistochemistry, cytokine, immune response.
Depositing User :	Symplectic Elements
Date Deposited :	26 Jul 2016 17:48
Last Modified :	16 Jan 2019 17:06
URI:	http://epubs.surrey.ac.uk/id/eprint/811463

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