Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers

Matta, C, Zhang, X, Liddell, S, Smith, JR and Mobasheri, A (2015) Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers BIOMARKERS, 20 (8). pp. 572-589.

Preview	Text Label free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes a technique for identification of membrane.pdf - Version of Record Available under License : See the attached licence file. Download (1MB) | Preview
Preview	Text (licence) SRI_deposit_agreement.pdf Available under License : See the attached licence file. Download (33kB) | Preview

Abstract

Context: There is insufficient knowledge about the chondrocyte membranome and its molecular composition. Objective: To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. Materials and methods: Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS. Results: A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms. Discussion: Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery.

Item Type:	Article
Subjects :	Veterinary Medicine
Divisions :	Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :	Name Email ORCID Matta, C Zhang, X Liddell, S Smith, JR Mobasheri, A
Date :	17 November 2015
DOI :	10.3109/1354750X.2015.1130191
Copyright Disclaimer :	This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http:// creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-com-mercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
Uncontrolled Keywords :	Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Toxicology, proteomics, chondrocyte, mass spectrometry, membranome, membrane protein extraction, equine, Triton X-114, Articular cartilage, DIFFERENTIATION IN-VITRO, MESENCHYMAL STEM-CELLS, MASS-SPECTROMETRY, PLASMA-MEMBRANE, CARTILAGE, OSTEOARTHRITIS, EXPRESSION, CHANNELS, MODEL, RECEPTORS
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	06 May 2016 17:43
Last Modified :	31 Oct 2017 18:17
URI:	http://epubs.surrey.ac.uk/id/eprint/810651

Actions (login required)

View Item

Downloads