Characterization of ascaris from Ecuador and Zanzibar.
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Sparks, AM, Betson, M, Oviedo, G, Sandoval, C, Cooper, PJ and Stothard, JR (2015) Characterization of ascaris from Ecuador and Zanzibar. J Helminthol, 89 (4). pp. 512-515.
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Abstract
To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis revealed the presence of A. lumbricoides in people and A. suum in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as A. suum. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species, A. suum being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar.
| Item Type: | Article | |||||||||||||||||||||
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| Subjects : | Veterinary Medicine | |||||||||||||||||||||
| Divisions : | Faculty of Health and Medical Sciences > School of Veterinary Medicine | |||||||||||||||||||||
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| Date : | July 2015 | |||||||||||||||||||||
| DOI : | 10.1017/S0022149X14000431 | |||||||||||||||||||||
| Copyright Disclaimer : | Copyright Cambridge University Press 2014. The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/ | |||||||||||||||||||||
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| Additional Information : | Copyright Cambridge University Press 2014. The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/ | |||||||||||||||||||||
| Depositing User : | Symplectic Elements | |||||||||||||||||||||
| Date Deposited : | 18 Mar 2016 18:26 | |||||||||||||||||||||
| Last Modified : | 31 Oct 2017 18:09 | |||||||||||||||||||||
| URI: | http://epubs.surrey.ac.uk/id/eprint/810234 |
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