High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products

Sakaridis, I, Ganopoulos, I, Argiriou, A and Tsaftaris, A (2013) High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products International Dairy Journal.

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Abstract

Identification of buffalo dairy products has become an important issue to ascertain product quality, consumer rights and absence of food-borne allergic reactions. A polymerase chain reaction (PCR) followed by a high resolution melting (HRM) analysis was developed and applied for species specific detection of bovine milk in nine different commercial buffalo dairy products. A specific buffalo 12S rRNA and a bovine d-loop primer pair, targeting the mitochondrial genome, were employed in a duplex PCR assay. The analysis developed was found capable of identifying the presence of bovine milk down to 1% in commercial buffalo milk products and also of quantifying the ratio of bovine into buffalo milk. HRM was proven to be a fast and accurate technique for a routine authentication testing of mozzarella and other buffalo milk products.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Sakaridis, I, Ganopoulos, I, Argiriou, A and Tsaftaris, A
Date :	8 January 2013
DOI :	10.1016/j.idairyj.2012.08.006
Depositing User :	Symplectic Elements
Date Deposited :	28 Mar 2017 10:57
Last Modified :	24 Jan 2020 12:44
URI:	http://epubs.surrey.ac.uk/id/eprint/809843

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