CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity.

Hamza, E, Steinbach, F and Marti, E (2012) CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol, 148 (1-2). pp. 139-144.

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Abstract

Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis caused by bites of midges from the genus Culicoides. We have shown previously that peripheral blood mononuclear cells (PBMC) from IBH-affected horses produce higher levels of IL-4 and lower levels of IL-10 and TGF-β1 than those from healthy horses, suggesting that IBH is associated with a reduced regulatory immune response. FoxP3 is a crucial marker of regulatory T cells (Tregs). Here we have determined the proportion of CD4(+)CD25(+)FoxP3(+) T cells by flow cytometry in PBMC directly after isolation or after stimulation with Culicoides extract or a control antigen (Tetanus Toxoid). There were no differences between healthy and IBH horses either in the proportion of FoxP3(+)CD4(+)CD25(+) cells in freshly isolated PBMC or in the following stimulation with Tetanus Toxoid. However, upon stimulation of PBMC with the allergen, expression of FoxP3 by CD4(+)CD25(+high) and CD4(+)CD25(+dim) cells was significantly higher in healthy than in IBH horses. Addition of recombinant IL-4 to PBMC from healthy horses stimulated with the allergen significantly decreased the proportion of FoxP3 expressing cells within CD4(+)CD25(+high). These results suggest that IBH is associated with a decreased number of allergen-induced Tregs. This could be a consequence of the increased IL-4 production by PBMC of IBH-affected horses.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Hamza, E, Steinbach, F and Marti, E
Date :	15 July 2012
DOI :	10.1016/j.vetimm.2011.05.033
Uncontrolled Keywords :	Animals, Cells, Cultured, Ceratopogonidae, Flow Cytometry, Forkhead Transcription Factors, Horse Diseases, Horses, Hypersensitivity, Insect Bites and Stings, Leukocytes, Mononuclear, T-Lymphocytes, Regulatory
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	28 Mar 2017 10:51
Last Modified :	24 Jan 2020 12:32
URI:	http://epubs.surrey.ac.uk/id/eprint/806872

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