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Investigation of protein imprinting in hydrogel-based molecularly imprinted polymers (HydroMIPs)

Hawkins, DM, Stevenson, D and Reddy, SM (2005) Investigation of protein imprinting in hydrogel-based molecularly imprinted polymers (HydroMIPs) In: 3rd International Workshop on Molecularly Imprinted Polmers, 2004-10-12 - 2004-10-15, Cardiff, WALES.

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We have developed a strategy to produce molecularly imprinted polymers based on polyacrylamide hydrogels for the selective imprinting of bovine haemoglobin (BHb). For the first time, we have explored in detail a variety of template removal strategies including varying ratios of sodium dodecylsulphate:acetic acid (SDS:AcOH) and also the use of a trypsin digest. The optimum ratio of SDS:AcOH was found to be a 10% (w/v):10% (v/v) for the most effective template removal. This resulted in >90% (imprinting efficiency) of re-loaded template (protein) molecule being selectively bound within the MIP. At 15%:15% of SDS:AcOH, although there was even more initial template removal, subsequent re-binding studies showed a decrease in imprinting efficiency (67.9%). Trypsin solutions were also used as a method of template removal. Up to 87.4% of template was reproducibly removed initially; however, the imprinting efficiency was only 20.4%. The high selectivity of the BHb HydroMIP to BHb compared with other structural analogues (namely cytochrome C and myoglobin) was successfully demonstrated.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Surrey research (other units)
Authors : Hawkins, DM, Stevenson, D and Reddy, SM
Date : 22 June 2005
DOI : 10.1016/j.aca.2005.01.052
Contributors :
ContributionNameEmailORCID SCIENCE BV,
Uncontrolled Keywords : Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, hydrogel, HydroMIP, molecular imprinting, proteins, polyacrylamide, molecularly imprinted polymers, MIP, RECOGNITION, PHASES
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Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:53
Last Modified : 23 Jan 2020 13:00

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