University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Optimisation of Mycobacterium bovis BCG Fermentation and Storage Survival

Pascoe, Jordan, Hendon-Dunn, Charlotte L., Birch, Colin P.D., Williams, Gareth A., Chambers, Mark A. and Bacon, Joanna (2020) Optimisation of Mycobacterium bovis BCG Fermentation and Storage Survival Pharmaceutics, 12 (9).

[img]
Preview
Text
pharmaceutics-12-00900.pdf - Version of Record
Available under License Creative Commons Attribution.

Download (800kB) | Preview

Abstract

Mycobacterium bovis Bacillus Calmette–Guérin (M. bovis BCG) was generated over a century ago for protection against Mycobacterium tuberculosis (Mtb) and is one the oldest vaccines still in use. The BCG vaccine is currently produced using a pellicle growth method, which is a complex and lengthy process that has been challenging to standardise. Fermentation for BCG vaccine production would reduce the complexity associated with pellicle growth and increase batch to batch reproducibility. This more standardised growth lends itself to quantification of the total number of bacilli in the BCG vaccine by alternative approaches, such as flow cytometry, which can also provide information about the metabolic status of the bacterial population. The aim of the work reported here was to determine which batch fermentation conditions and storage conditions give the most favourable outcomes in terms of the yield and stability of live M. bovis BCG Danish bacilli. We compared different media and assessed growth over time in culture, using total viable counts, total bacterial counts, and turbidity throughout culture. We applied fluorescent viability dyes and flow cytometry to measure real-time within-culture viability. Culture samples were stored in different cryoprotectants at different temperatures to assess the effect of these combined conditions on bacterial titres. Roisin’s minimal medium and Middlebrook 7H9 medium gave comparable, high titres in fermenters. Flow cytometry proved to be a useful tool for enumeration of total bacterial counts and in the assessment of within-culture cell viability and cell death. Of the cryoprotectants evaluated, 5% (v/v) DMSO showed the most significant positive effect on survival and reduced the negative effects of low temperature storage on M. bovis BCG Danish viability. In conclusion, we have shown a reproducible, more standardised approach for the production, evaluation, and storage of high titre, viable, BCG vaccine.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Pascoe, Jordan
Hendon-Dunn, Charlotte L.
Birch, Colin P.D.
Williams, Gareth A.
Chambers, Mark A.m.chambers@surrey.ac.uk
Bacon, Joanna
Date : 22 September 2020
DOI : 10.3390/pharmaceutics12090900
Copyright Disclaimer : © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords : BCG; Fermentation; Flow cytometry; Calcein violet; Sytox green; Cryoprotectant
Additional Information : This article belongs to the Special Issue Tuberculosis Vaccine Research and Development - https://www.mdpi.com/journal/pharmaceutics/special_issues/TB_vaccine
Depositing User : Clive Harris
Date Deposited : 07 Oct 2020 14:25
Last Modified : 07 Oct 2020 14:25
URI: http://epubs.surrey.ac.uk/id/eprint/858702

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800