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Loss of phenotypic inheritance associated with ydcI mutation leads to increased frequency of small, slow persisters in Escherichia coli

Hingley-Wilson, Suzanne M., Ma, Nan, Hu, Yin, Casey, Rosalyn, Bramming, Anders, Curry, Richard J., Tang, Hongying Lilian, Wu, Huihai, Butler, Rachel E., Jacobs, William R. , Rocco, Andrea and McFadden, Johnjoe (2020) Loss of phenotypic inheritance associated with ydcI mutation leads to increased frequency of small, slow persisters in Escherichia coli Proceedings of the National Academy of Sciences, 117 (8). pp. 4152-4157.

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Abstract

Persistence, the phenomenon whereby a small subpopulation of bacterial cells survive sterilization, prolongs antibiotic treatment and contributes to the development of genetic antimicrobial drug resistance (AMR). In this study we performed single-cell tracking of wild-type and high-persister mutant strains of Escherichia coli to identify factors that correlate with persistence. We found, as expected, persistence correlated with slow growth, but also with small birth size. We investigated intergenerational (mother–daughter) and intragenerational (sister–sister) phenotypic inheritance of growth parameters and discovered the mutant phenotype was associated with lower levels of phenotypic inheritance and identified the gene responsible, the transcription factor ydcI. Targeting pathways involved in persistence could reveal approaches to impeding persistence and the development of AMR.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Computer Science
Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Hingley-Wilson, Suzanne M.s.hingley-wilson@surrey.ac.uk
Ma, Nan
Hu, Yinyin.hu@surrey.ac.uk
Casey, Rosalynrosalyn.casey@surrey.ac.uk
Bramming, Anders
Curry, Richard J.
Tang, Hongying LilianH.Tang@surrey.ac.uk
Wu, HuihaiH.Wu@surrey.ac.uk
Butler, Rachel E.R.Shrimpton@surrey.ac.uk
Jacobs, William R.
Rocco, AndreaA.Rocco@surrey.ac.uk
McFadden, JohnjoeJ.Mcfadden@surrey.ac.uk
Date : 25 February 2020
Funders : Biotechnology and Biological Sciences Research Council (BBSRC), Royal Society, National Institutes of Health, Medical Research Council (MRC), Natural Environment Research Council (NERC)
DOI : 10.1073/pnas.1914741117
Copyright Disclaimer : Copyright © 2020 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
Uncontrolled Keywords : Persistence; Antibiotic resistance; Phenotypic; Microbiology; Systems biology
Additional Information : This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1914741117/-/DCSupplemental. Data deposition: Individual sequencing read data for the E. coli HipQ mutant and parental strain are publicly available at National Center for Biotechnology Information (NCBI) Biosample, https://www.ncbi.nlm.nih.gov/biosample (accession nos. SAMN13648605 [E.coli HipQ mutant strain] and SAMN13648604 [E. coli parental strain]).
Depositing User : Clive Harris
Date Deposited : 06 Oct 2020 13:46
Last Modified : 06 Oct 2020 13:46
URI: http://epubs.surrey.ac.uk/id/eprint/858675

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