Investigating the effects of nisin and free fatty acid combined treatment on Listeria monocytogenes inactivation

Zhou, Jiacheng, Velliou, Eirini and Hong, Seok Hoon (2020) Investigating the effects of nisin and free fatty acid combined treatment on Listeria monocytogenes inactivation LWT - Food Science and Technology, 133, 110115.

Text NisFA Listeria R1 v1.docx - Accepted version Manuscript Restricted to Repository staff only until 1 December 2021. Download (580kB)

Abstract

The FDA-approved antimicrobial peptide nisin has been used to prevent contamination of food products from Gram-positive pathogens including Listeria monocytogenes (L. monocytogenes). However, some L. monocytogenes strains are tolerant or resistant to nisin treatment at a dose that would normally be lethal. Therefore, alternative or combined antimicrobial treatment strategies are required to effectively control L. monocytogenes contaminations. In this study, we investigate whether combination treatment with nisin and fatty acids can overcome the nisin resistance of L. monocytogenes by monitoring cell survival and biofilm inactivation of two strains (ATCC15313 serotype 1/2a and ATCC19115 serotype 4b). Lauric acid and N-tridecanoic acid were chosen because they exhibit antimicrobial activities. First, we observed that nisin (22.5 μg/mL) rapidly killed cells with up to a 6-log reduction after 3 h, but treatment for prolonged periods (i.e., up to 24 h) caused the cells to recover growth via the development of either inherent nisin resistance or temporary nisin tolerance. However, co-treatment of nisin and each fatty acid significantly decreased the cell survival and hindered the development of nisin resistance. In addition, the co-treatment effectively inactivated cells in biofilms. The combined antimicrobial treatment may be promising approach in the food processing environment, where Listeria contamination occurs.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :	Name Email ORCID Zhou, Jiacheng Velliou, Eirini e.velliou@surrey.ac.uk Hong, Seok Hoon
Date :	November 2020
DOI :	10.1016/j.lwt.2020.110115
Copyright Disclaimer :	© 2020 Elsevier Ltd. All rights reserved.
Uncontrolled Keywords :	Listeria monocytogenes survival; Nisin resistance; BiofilmLauric acid; N-tridecanoic acid
Depositing User :	Diane Maxfield
Date Deposited :	01 Oct 2020 12:39
Last Modified :	01 Oct 2020 12:39
URI:	http://epubs.surrey.ac.uk/id/eprint/858609

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