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Influence of tissue fixation on depth-resolved birefringence of oral cavity tissue samples

Karnowski, Karol, Li, Qingyun, Poudyal, Anima, Villiger, Martin, Farah, Camile S. and Sampson, David D. (2020) Influence of tissue fixation on depth-resolved birefringence of oral cavity tissue samples Journal of Biomedical Optics, 25 (09), 096003.

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Abstract

Significance: To advance our understanding of the contrast observed when imaging with polarization-sensitive optical coherence tomography (PS-OCT) and its correlation with oral cancerous pathologies, a detailed comparison with histology provided via ex vivo fixed tissue is required. The effects of tissue fixation, however, on such polarization-based contrast have not yet been investigated.

Aim: A study was performed to assess the impact of tissue fixation on depth-resolved (i.e., local) birefringence measured with PS-OCT.

Approach: A PS-OCT system based on depth-encoded polarization multiplexing and polarization-diverse detection was used to measure the Jones matrix of a sample. A wide variety of ex vivo samples were measured freshly after excision and 24 h after fixation, consistent with standard pathology. Some samples were also measured 48 h after fixation.

Results: The tissue fixation does not diminish the birefringence contrast. Statistically significant changes were observed in 11 out of 12 samples; these changes represented an increase in contrast, overall, by 11% on average.

Conclusions: We conclude that the fixed samples are suitable for studies seeking a deeper understanding of birefringence contrast in oral tissue pathology. The enhancement of contrast removes the need to image immediately postexcision and will facilitate future investigations with PS-OCT and other advanced polarization-sensitive microscopy methods, such as mapping of the local optic axis with PS-OCT and PS-optical coherence microscopy.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences
Surrey research (other units)
Authors :
NameEmailORCID
Karnowski, Karol
Li, Qingyun
Poudyal, Anima
Villiger, Martin
Farah, Camile S.
Sampson, David D.d.sampson@surrey.ac.uk
Date : 10 September 2020
Funders : Australian Dental Research Foundation, Polish National Agency for Academic Exchange, European Union’s Horizon 2020, Australian Government International Research Training Program (RTP), National Institutes of Health
DOI : 10.1117/1.JBO.25.9.096003
Copyright Disclaimer : © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Uncontrolled Keywords : Biomedical imaging; Tissue fixation; Optical coherence tomography; Oral tissue
Depositing User : Clive Harris
Date Deposited : 10 Sep 2020 09:52
Last Modified : 10 Sep 2020 09:52
URI: http://epubs.surrey.ac.uk/id/eprint/858556

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