DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems

Henslee, Erin A., Dunlop, Carina, de Mel, Christine M., Carter, Emily, Abdallat, Rula, Camelliti, Patrizia and Labeed, Fatima (2020) DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems Scientific Reports, 10, 14603.

Preview

Text Henslee et al Scientific Reports 2020 FINAL REVISION.pdf - Accepted version Manuscript Download (1MB) | Preview

Abstract

It is known that cells grown in 3D are more tolerant to drug treatment than those grown in dispersion but the mechanism for this is still not clear; cells grown in 3D have opportunities to develop inter-cell communication, but are also closely packed which may impede diffusion. In this study we examine methods for dielectrophoresis-based cell aggregation of both suspension and adherent cell lines and compare the effect of various drugs on cells grown in 3D and 2D. Comparing viability of pharmacological interventions on 3D cell clusters against both suspension cells and adherent cells grown in monolayer, as well as against a unicellular organism with no propensity for intracellular communication, we suggest that 3D aggregates of adherent cells, compared to suspension cells, show a substantially different drug response to cells grown in monolayer, which increases as the IC50 is approached. Further, a mathematical model of the system for each agent demonstrates that changes to drug response are due to inherent changes in the system of adherent cells from the 2D to 3D state. Finally, differences within electrophysiological membrane properties of the adherent cell type suggest this parameter plays an important role in the differences found in the 3D drug response.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :	Name Email ORCID Henslee, Erin A. Dunlop, Carina c.dunlop@surrey.ac.uk de Mel, Christine M. Carter, Emily Abdallat, Rula r.abdallat@surrey.ac.uk Camelliti, Patrizia p.camelliti@surrey.ac.uk Labeed, Fatima F.Labeed@surrey.ac.uk
Date :	3 September 2020
Funders :	Engineering and Physical Sciences Research Council (EPSRC)
DOI :	10.1038/s41598-020-71265-7
Grant Title :	EPSRC Grant
Copyright Disclaimer :	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Additional Information :	Embargo OK Metadata Pending
Depositing User :	James Marshall
Date Deposited :	15 Jul 2020 15:50
Last Modified :	06 Oct 2020 12:39
URI:	http://epubs.surrey.ac.uk/id/eprint/858219

Actions (login required)

View Item

Downloads