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Introducing Porosity in Colloidal Biocoatings to Increase Bacterial Viability

Chen, Yuxiu, Krings, Simone, Booth, Joshua R., Bon, Stefan A.F, Hingley-Wilson, Suzie and Keddie, Joseph (2020) Introducing Porosity in Colloidal Biocoatings to Increase Bacterial Viability Biomacromolecules.

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Abstract

A biocoating confines non-growing, metabolically-active bacteria within a synthetic colloidal polymer (i.e. latex) film. Bacteria encapsulated inside biocoatings can perform useful functions, such as a biocatalyst in wastewater treatment. A biocoating needs to have high a permeability to allow a high rate of mass transfer for rehydration and the transport of both nutrients and metabolic products. It therefore requires an interconnected porous structure. Tuning the porosity architecture is a challenge. Here, we exploited rigid tubular nanoclays (halloysite) and non-toxic latex particles (with a relatively high glass transition temperature) as the colloidal “building blocks” to tailor the porosity inside biocoatings containing Escherichia coli bacteria as a model organism. Electron microscope images revealed inefficient packing of the rigid nanotubes and proved the existence of nanovoids along the halloysite/polymer interfaces. Single-cell observations using confocal laser scanning microscopy provided evidence for metabolic activity of the E. coli within the biocoatings through the expression of yellow fluorescent protein. A custom-built apparatus was used to measure the permeability of a fluorescein sodium salt in the biocoatings. Whereas there was no measurable permeability in a coating made from only latex particles, the permeability coefficient of the composite biocoatings increased with increasing halloysite content up to a value of 110-4 m h-1. The effects of this increase in permeability was demonstrated through a specially-developed resazurin reduction assay. Bacteria encapsulated in halloysite composite biocoatings had statistically significant higher metabolic activities in comparison to bacteria encapsulated in a non-optimized coating made from latex particles alone.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Physics
Authors :
NameEmailORCID
Chen, Yuxiuyuxiu.chen@surrey.ac.uk
Krings, Simones.krings@surrey.ac.uk
Booth, Joshua R.
Bon, Stefan A.F
Hingley-Wilson, Suzies.hingley-wilson@surrey.ac.uk
Keddie, JosephJ.Keddie@surrey.ac.uk
Date : 25 June 2020
Funders : The Leverhulme Trust, Merck KGaA
Projects : Research Project Grant - The Leverhulme Trust
Additional Information : Embargo OK Metadata Pending
Depositing User : James Marshall
Date Deposited : 02 Jul 2020 09:37
Last Modified : 02 Jul 2020 09:46
URI: http://epubs.surrey.ac.uk/id/eprint/858121

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