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Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy

Park, HyunJoo, Hong, Sung-Hee, Kim, Kyoohyun, Cho, Shin-Hyeong, Lee, Won-Ja, Kim, Youngchan, Lee, Sang-Eun and Park, YongKeun (2015) Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy Scientific Reports, 5 (1), 10827.

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Abstract

Babesia microti causes “emergency” human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm-RBCs. 3-D refractive index maps of individual RBCs and in situ parasite vacuoles are imaged, from which total contents and concentration of dry mass are also precisely quantified. In addition, we examine the dynamic membrane fluctuation of Bm-RBCs, which provide information on cell membrane deformability.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Park, HyunJoo
Hong, Sung-Hee
Kim, Kyoohyun
Cho, Shin-Hyeong
Lee, Won-Ja
Kim, Youngchanyoungchan.kim@surrey.ac.uk
Lee, Sang-Eun
Park, YongKeun
Date : 3 June 2015
DOI : 10.1038/srep10827
Copyright Disclaimer : This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Depositing User : James Marshall
Date Deposited : 29 Jun 2020 09:49
Last Modified : 29 Jun 2020 09:49
URI: http://epubs.surrey.ac.uk/id/eprint/858081

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