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Exploration of Matrix Effects in Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Cisplatin Treated Tumours

Greenhalgh, Calum J., Karekla, Ellie, Miles, Gareth J., Powley, Ian, Costa, Catia, De Jesus, Janella, Bailey, Melanie, Pritchard, Catrin, MacFarlane, Marion, Pringle, J. Howard and Managh, Amy (2020) Exploration of Matrix Effects in Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Cisplatin Treated Tumours Analytical Chemistry.

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Abstract

The use of a low aerosol dispersion ablation chamber within a LA-ICP-MS set up allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report the distribution of platinum at the cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses of cisplatin. This revealed for the first time a correlation between the platinum signal and the presence of carbon deposits within lung tissue. We show how complementary ion beam analysis techniques, particle induced X-ray emission (PIXE) and elastic backscattering spectrometry (EBS) can be used to explore potential matrix effects in LA-ICP-MS data. For these samples, it was confirmed that the enhancement was unlikely to have resulted from a matrix effect alone. Thus, the presence of carbon deposits within tissue has potential implications for the effective distribution of the cisplatin drug.

Item Type: Article
Divisions : Library and Learning Support
Authors :
NameEmailORCID
Greenhalgh, Calum J.
Karekla, Ellie
Miles, Gareth J.
Powley, Ian
Costa, Catiac.d.costa@surrey.ac.uk
De Jesus, Janellaj.dejesus@surrey.ac.uk
Bailey, MelanieM.Bailey@surrey.ac.uk
Pritchard, Catrin
MacFarlane, Marion
Pringle, J. Howard
Managh, Amy
Date : 17 June 2020
Funders : Loughborough University Funded PhD Studentship, Medical Research Council (MRC) Doctoral Training Grant, Leicester Experimental Cancer Medicine Centre, Royal Society, EPSRC Pump Primer Grant
DOI : 10.1021/acs.analchem.0c01347
Grant Title : MRC Toxicology Unit Grant
Copyright Disclaimer : Copyright © 2020 American Chemical Society
Additional Information : Embargo OK Metadata incomplete: version online is in-print, not final version.
Depositing User : James Marshall
Date Deposited : 23 Jun 2020 14:23
Last Modified : 23 Jun 2020 14:23
URI: http://epubs.surrey.ac.uk/id/eprint/858048

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