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Pan-Genomic Analysis of Clostridium botulinum Group II (Non-Proteolytic C. botulinum) Associated with Foodborne Botulism and Isolated from the Environment

Brunt, Jason, van Vliet, Arnoud H. M., Stringer, Sandra C., Carter, Andrew T., Lindström, Miia and Peck, Michael W. (2020) Pan-Genomic Analysis of Clostridium botulinum Group II (Non-Proteolytic C. botulinum) Associated with Foodborne Botulism and Isolated from the Environment Toxins, 12 (5), 306.

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Abstract

The neurotoxin formed by Clostridium botulinum Group II is a major cause of foodborne botulism, a deadly intoxication. This study aims to understand the genetic diversity and spread of C. botulinum Group II strains and their neurotoxin genes. A comparative genomic study has been conducted with 208 highly diverse C. botulinum Group II strains (180 newly sequenced strains isolated from 16 countries over 80 years, 28 sequences from Genbank). Strains possessed a single type B, E, or F neurotoxin gene or were closely related strains with no neurotoxin gene. Botulinum neurotoxin subtype variants (including novel variants) with a unique amino acid sequence were identified. Core genome single-nucleotide polymorphism (SNP) analysis identified two major lineages—one with type E strains, and the second dominated by subtype B4 strains with subtype F6 strains. This study revealed novel details of population structure/diversity and established relationships between whole-genome lineage, botulinum neurotoxin subtype variant, association with foodborne botulism, epidemiology, and geographical source. Additionally, the genome sequences represent a valuable resource for the research community (e.g., understanding evolution of C. botulinum and its neurotoxin genes, dissecting key aspects of C. botulinum Group II biology). This may contribute to improved risk assessments and the prevention of foodborne botulism.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Brunt, Jason
van Vliet, Arnoud H. M.a.vanvliet@surrey.ac.uk
Stringer, Sandra C.
Carter, Andrew T.
Lindström, Miia
Peck, Michael W.
Date : 8 May 2020
Funders : Biotechnology and Biological Sciences Research Council (BBSRC), European Research Council (ERC)
DOI : 10.3390/toxins12050306
Copyright Disclaimer : This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Uncontrolled Keywords : Clostridium botulinum; Botulism; Foodborne; Neurotoxin; Non-proteolytic
Depositing User : Clive Harris
Date Deposited : 10 Jun 2020 10:06
Last Modified : 10 Jun 2020 10:06
URI: http://epubs.surrey.ac.uk/id/eprint/857318

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