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HDL-apoA-I kinetics in response to 16 wk of exercise training in men with nonalcoholic fatty liver disease

Whyte, Martin B., Shojaee-Moradie, Fariba, Sharaf, Sharaf E., Cuthbertson, Daniel J., Kemp, Graham J., Barrett, Mark, Jackson, Nicola C., Herring, Roselle A., Wright, John, Thomas, E. Louise , Bell, Jimmy and Umpleby, A. Margot (2020) HDL-apoA-I kinetics in response to 16 wk of exercise training in men with nonalcoholic fatty liver disease American Journal of Physiology-Endocrinology and Metabolism, 318 (6). E839-E847.

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Abstract

Nonalcoholic fatty liver disease (NAFLD) is characterized by low-circulating concentration of high-density lipoprotein cholesterol (HDL-C) and raised triacylglycerol (TAG). Exercise reduces hepatic fat content, improves insulin resistance and increases clearance of very-low-density lipoprotein-1 (VLDL1). However, the effect of exercise on TAG and HDL-C metabolism is unknown. We randomized male participants to 16 wk of supervised, moderate-intensity aerobic exercise (n = 15), or conventional lifestyle advice (n = 12). Apolipoprotein A-I (apoA-I) and VLDL-TAG and apolipoprotein B (apoB) kinetics were investigated using stable isotopes (1-[13C]-leucine and 1,1,2,3,3-2H5 glycerol) pre- and postintervention. Participants underwent MRI/spectroscopy to assess changes in visceral fat. Results are means ± SD. At baseline, there were no differences between exercise and control groups for age (52.4 ± 7.5 vs. 52.8 ± 10.3 yr), body mass index (BMI: 31.6 ± 3.2 vs. 31.7 ± 3.6 kg/m2), and waist circumference (109.3 ± 7.5 vs. 110.0 ± 13.6 cm). Percentage of liver fat was 23.8 (interquartile range 9.8–32.5%). Exercise reduced body weight (101.3 ± 10.2 to 97.9 ± 12.2 kg; P ˂ 0.001) and hepatic fat content [from 19.6%, interquartile range (IQR) 14.6–36.1% to 8.9% (4.4–17.8%); P = 0.001] and increased the fraction HDL-C concentration (measured following ultracentrifugation) and apoA-I pool size with no change in the control group. However, plasma and VLDL1-TAG concentrations and HDL-apoA-I fractional catabolic rate (FCR) and production rate (PR) did not change significantly with exercise. Both at baseline (all participants) and after exercise there was an inverse correlation between apoA-I pool size and VLDL-TAG and -apoB pool size. The modest effect of exercise on HDL metabolism may be explained by the lack of effect on plasma and VLDL1-TAG.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Whyte, Martin B.m.b.whyte@surrey.ac.uk
Shojaee-Moradie, Fariba
Sharaf, Sharaf E.
Cuthbertson, Daniel J.
Kemp, Graham J.
Barrett, Mark
Jackson, Nicola C.
Herring, Roselle A.
Wright, John
Thomas, E. Louise
Bell, Jimmy
Umpleby, A. Margot
Date : 1 June 2020
DOI : 10.1152/ajpendo.00019.2020
Copyright Disclaimer : © 2020 the American Physiological Society
Uncontrolled Keywords : NALFD; Exercise; HDL
Additional Information : 12 month embargo. No further action
Depositing User : Clive Harris
Date Deposited : 22 May 2020 16:00
Last Modified : 22 May 2020 16:00
URI: http://epubs.surrey.ac.uk/id/eprint/856977

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