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Immunomodulatory Intervention to Control Classical Swine Fever.

Soule, Olubukola Ashabi. (2013) Immunomodulatory Intervention to Control Classical Swine Fever. Doctoral thesis, University of Surrey (United Kingdom)..

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Abstract

Classical swine fever virus (CSFV) causes a serious disease of pigs that is a major threat to pork production industries worldwide. Due to the limitations of available vaccines there is a need for alternative strategies to aid CSF outbreak control. One such alternative could be through the use of type I interferon (IFN). During the acute phase of CSFV infection, high levels of systemic porcine IFNα (PoIFNα) are detected in pigs and one recombinant PoIFNα subtype has been reported to have anti-viral activities against CSFV in vitro. The aim of this study was to express and characterize the full panel of recombinant PoIFN-α subtypes and to establish an in vitro model to assess their anti-CSFV activity if given prophylactically. All 17 PoIFN-α subtypes were cloned, expressed in Chinese hamster ovary cells and purified. The biological activity of each subtype was confirmed using a VSV bioassay. Following the optimisation of an infection model using a commercial preparation of PoIFN-α, differential activity was observed between subtypes following treatment of porcine monocytes prior to infection with CSFV. The subtypes were subsequently grouped as having high, medium or low anti-CSFV activity. The potency of representative members of the active subtypes was investigated over a 72hr pre-treatment assay which confirmed PoIFNα12 as the most potent subtype against CSFV in vitro. An additive effect was observed when equal ratios of subtypes within an activity group were used in combination. The expression of each subtype was then assessed during the course of a primary CSFV infection in vivo. When comparing the relative patterns of expression of the PoIFNα subtypes in individual pigs, several subtypes appeared to be expressed at higher levels. However, the only consistent increase post CSFV infection was that of PoIFNα8. In conclusion, this study has shown that PoIFNα subtypes possess differential anti-CSFV activities in vitro, and that PoIFNα12 represents a possible candidate for investigation as a me taphylactic treatment against CSFV infection.

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors : Soule, Olubukola Ashabi.
Date : 2013
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 2013.
Depositing User : EPrints Services
Date Deposited : 14 May 2020 14:17
Last Modified : 14 May 2020 14:23
URI: http://epubs.surrey.ac.uk/id/eprint/856561

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