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Mechanisms of Lipid Oxidation and Safety Assessment in Underutilised Fish Species.

Sarkardel, Samiramis. (2005) Mechanisms of Lipid Oxidation and Safety Assessment in Underutilised Fish Species. Doctoral thesis, University of Surrey (United Kingdom)..

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Abstract

The aim of this thesis was to develop a food recipe for the developing countries using underutilised fish species. The food was freeze-dried and stored at 22&amp;deg;C with and without antioxidants. Lipid oxidation and safety of the fish used in the recipe were assessed. The lipid and protein content were at 5% and 19.9% respectively for mackerel and at 2% and 21.6% respectively for horse mackerel. The moisture content was approximately 71% for both fish. Raman spectra of oil extracted from freeze-dried mackerel and horse mackerel revealed significant reductions in the intensity of bands associated with CH2 stretches and C=O ester stretches and increase in the intensity of the bands at 3011 cm-1 and 2960-2850 cm-1 suggesting alterations in lipid structure involving CH groups, as a result of lipid oxidation confirmed by peroxide value determinations. Combination of vitamins E + C + citric acid (250; 250; 100 ppm) was shown to be the most effective in slowing lipid oxidation in fish (P < 0.05) and in food products (P < 0.01). A rapid decrease (P <0.01) in solubility of myofibrillar proteins was detected in the control samples. Gel electrophoresis of myofibrillar proteins of freeze-dried mackerel showed a gradual loss of the myosin band in control samples. Addition of methyl linoleate (ML) or mackerel oil oxidized under UV light for 72 hours caused a substantial drop in the percentage of LDH release. Combination of vitamins E + C + citric acid (250; 250; 100 ppm) as well as rosemary (250 ppm) showed a significant decrease in the percentage of LDH release compared with the control (without antioxidants) group (P < 0.01 and P < 0.05 respectively). The data showed that fish from African and Asian marine waters contain PCDD/Fs at levels significantly below the limit set by the European Commission. An assessment of dietary exposure to PCBs and Dioxins showed a daily intake per capita of these contaminants below the tolerable daily intake (TDI) and tolerable monthly intake for dioxins established by World Health Organisation.

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors : Sarkardel, Samiramis.
Date : 2005
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 2005.
Depositing User : EPrints Services
Date Deposited : 14 May 2020 14:03
Last Modified : 14 May 2020 14:08
URI: http://epubs.surrey.ac.uk/id/eprint/856444

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