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Induction of Cytochrome P-450 in in vitro Hepatocyte Culture Systems.

Parker, Gerald Leslie. (1985) Induction of Cytochrome P-450 in in vitro Hepatocyte Culture Systems. Doctoral thesis, University of Surrey (United Kingdom)..

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Hepatocyte isolation procedures were developed for tissue derived from Rat, Mouse, Man, Guinea pig, and Hamster. Hepatocyte cultures were derived from the above species, with the exception of Man, and successfully maintained for four days. The cultures were assessed for the ability to metabolise xenobiotics, and exposed to classical inducing agents in vitro. Induction of the cytochrome P-450 monooxygenase system was demonstrated following exposure to phenobarbitone and β-naphthoflavone, and the nature of induction characterised by use of specific enzyme substrates and inhibitors.

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors : Parker, Gerald Leslie.
Date : 1985
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 1985.
Depositing User : EPrints Services
Date Deposited : 06 May 2020 14:23
Last Modified : 06 May 2020 14:30

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