University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Platelet-derived Transforming Growth Factor-β1 promotes keratinocyte proliferation in cutaneous wound healing

Chong, Deborah L.H, Trinder, Sarah, Labelle, Myriam, Rodriguez-Justo, Manuel, Hughes, Sian, Holmes, Alan M., Scotton, Chris J. and Porter, Joanna C. (2020) Platelet-derived Transforming Growth Factor-β1 promotes keratinocyte proliferation in cutaneous wound healing Journal of Tissue Engineering and Regenerative Medicine.

[img] Text
Chong_et_al-2020-Journal_of_Tissue_Engineering_and_Regenerative_Medicine.pdf - Accepted version Manuscript
Restricted to Repository staff only until 15 February 2021.

Download (971kB)

Abstract

Platelets are a recognised potent source of Transforming Growth Factor-1 (TGF1), a cytokine known to promote wound healingand regenerationby stimulating dermal fibroblast proliferation and extracellular matrix deposition. Platelet lysate has been advocated as a novel personalised therapeutic to treat persistent wounds, although the precise platelet-derived growth factors responsible for these beneficial effects have not been fully elucidated. The aim of thisstudy was to investigate the specific role of platelet-derived TGF1in cutaneous wound healing.Using a transgenic mouse with a targeted deletion of TGF1in megakaryocytes and platelets (TGFβ1fl/fl.PF4-Cre), we show for the first-time platelet-derived TGF1 contributes to epidermal and dermal thickening and cellular turnover after excisional skin wounding. In vitrostudies demonstrate human dermal fibroblasts stimulated with platelet lysate containing high levels of platelet-derived TGF1 did not exhibit enhanced collagen deposition or proliferation, suggestingthat platelet-derived TGF1 is not a key promoter of these wound healing processes.Interestingly, human keratinocytes displayed enhanced TGF1-driven proliferation in response to platelet lysate, reminiscent of our in vivofindings. In summary, our novel findings define andemphasiseanimportant roleof platelet-derived TGF1in epidermal remodellingand regenerationprocessesduring cutaneous wound healing.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Chong, Deborah L.H
Trinder, Sarahs.trinder@surrey.ac.uk
Labelle, Myriam
Rodriguez-Justo, Manuel
Hughes, Sian
Holmes, Alan M.
Scotton, Chris J.
Porter, Joanna C.
Date : 18 February 2020
Funders : Department of Health’s NIHR Biomedical Research Centre, Medical Research Council (MRC)
OA Location : https://onlinelibrary.wiley.com/doi/abs/10.1002/term.3022
Grant Title : Medical Research Council (MRC)
Copyright Disclaimer : This article is protected by copyright. All rights reserved.
Uncontrolled Keywords : Blood platelets; Epidermis; Keratinocytes; Platelet-rich plasma; Skin;Transforming Growth Factor Beta; Transgenic mouse; Wound healing
Depositing User : James Marshall
Date Deposited : 20 Feb 2020 14:33
Last Modified : 20 Feb 2020 14:33
URI: http://epubs.surrey.ac.uk/id/eprint/853805

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800