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In vivo brain imaging with multimodal optical coherence microscopy in a mouse model of thromboembolic photochemical stroke

Dolezyczek, Hubert, Tamborski, Szymon, Majka, Piotr, Sampson, Danuta, Wojtkowski, Maciej, Wilczyński, Grzegorz, Szkulmowski, Maciej and Malinowska, Monika (2020) In vivo brain imaging with multimodal optical coherence microscopy in a mouse model of thromboembolic photochemical stroke Neurophotonics.

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Abstract

We used a new multimodal imaging system that combines optical coherence microscopy and brightfield microscopy. Using this in vivo brain monitoring approach and cranial window implantation, we three-dimensionally visualized the vascular network during thrombosis, with high temporal (18 s) and spatial (axial, 2.5 μm; lateral, 2.2 μm) resolution.We used a modified mouse model of photochemical thromboembolic stroke in order to more accurately parallel human stroke. Specifically, we applied green laser illumination to focally occlude a branch of the middle cerebral artery. Despite the recanalization of the superficial arteries at 24 h after stroke, no blood flow was detected in the small vessels within deeper regions. Moreover, after 24 h of stroke progression, scattering signal enhancement was observed within the stroke region. We also evaluated the infarct extent and shape histologically. In summary, we present a novel approach for real-time mouse brain monitoring and ischemic variability analysis. This multimodal imaging method permits the analysis of thrombosis progression and reperfusion. Additionally and importantly, the system could be used to study the effect of poststroke drug treatments on blood flow in small arteries and capillaries of the brain.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :
NameEmailORCID
Dolezyczek, Hubert
Tamborski, Szymon
Majka, Piotr
Sampson, Danutads0062@surrey.ac.uk
Wojtkowski, Maciej
Wilczyński, Grzegorz
Szkulmowski, Maciej
Malinowska, Monika
Date : 22 January 2020
Copyright Disclaimer : © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Uncontrolled Keywords : optical coherence microscopy; photochemical thromboembolic stroke; angiography; cranial window; in vivo mouse brain imaging; cerebral blood flow.
Depositing User : James Marshall
Date Deposited : 03 Feb 2020 10:13
Last Modified : 03 Feb 2020 10:13
URI: http://epubs.surrey.ac.uk/id/eprint/853547

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