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Norovirus infection results in eIF2α independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation

Brocard, Michele, Ladevaia, Valentina, Klein, Philipp, Hall, Belinda, Lewis, Glenys, Lu, Jia, Burke, James, Willcocks, Margaret, Parker, Roy, Goodfellow, Ian G. , Ruggieri, Alessia and Locker, Nicolas (2020) Norovirus infection results in eIF2α independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation PLoS Pathogens.

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Abstract

Viral infections impose major stress on the host cell. In response, stress pathways can rapidly deploy defence mechanisms by shutting off the protein synthesis machinery and triggering the accumulation of mRNAs into stress granules to limit the use of energy and nutrients. Because this threatens viral gene expression, viruses need to evade these pathways to propagate. Human norovirus is responsible for gastroenteritis outbreaks worldwide. Here we examined how norovirus interacts with the eIF2α signaling axis controlling translation and stress granules. While norovirus infection represses host cell translation, our mechanistic analyses revealed that eIF2α signaling mediated by the stress kinase GCN2 is uncoupled from translational stalling. Moreover, infection results in a redistribution of the RNA-binding protein G3BP1 to replication complexes and remodelling of its interacting partners, allowing the avoidance from canonical stress granules. These results define novel strategies by which norovirus undergo efficient replication whilst avoiding the host stress response and manipulating the G3BP1 interactome.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Brocard, Michelem.brocard@surrey.ac.uk
Ladevaia, Valentina
Klein, Philipp
Hall, BelindaB.S.Hall@surrey.ac.uk
Lewis, Glenys
Lu, Jia
Burke, James
Willcocks, Margaret
Parker, Roy
Goodfellow, Ian G.
Ruggieri, Alessia
Locker, NicolasN.Locker@surrey.ac.uk
Date : 6 January 2020
Copyright Disclaimer : © 2020 Brocard et al.
Depositing User : James Marshall
Date Deposited : 30 Jan 2020 09:56
Last Modified : 30 Jan 2020 09:56
URI: http://epubs.surrey.ac.uk/id/eprint/853490

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