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Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis.

Hoettges, Kai, Henslee, Erin, Torcal Serrano, Ruth M., Jabr, Rita, Abdallat, Rula, Beale, Andrew, Waheed, Abdul, Camelliti, Patrizia, Fry, Christopher, Van Der Veen, Daan , Labeed, Fatima and Hughes, Michael (2019) Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Scientific Reports, 9, 19153.

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Abstract

Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
NameEmailORCID
Hoettges, KaiK.Hoettges@surrey.ac.uk
Henslee, Erine.henslee@surrey.ac.uk
Torcal Serrano, Ruth M.
Jabr, Ritarita.jabr@surrey.ac.uk
Abdallat, Rular.abdallat@surrey.ac.uk
Beale, Andrewa.beale@surrey.ac.uk
Waheed, Abdul
Camelliti, Patriziap.camelliti@surrey.ac.uk
Fry, ChristopherC.H.Fry@surrey.ac.uk
Van Der Veen, DaanD.Vanderveen@surrey.ac.uk
Labeed, FatimaF.Labeed@surrey.ac.uk
Hughes, MichaelM.Hughes@surrey.ac.uk
Date : 16 December 2019
Funders : South East of England Development Agency, Finance South East, Labtech International
DOI : https://doi.org/10.1038/s41598-019-55579-9
Copyright Disclaimer : Copyright © 2019, Springer Nature
Depositing User : James Marshall
Date Deposited : 24 Jan 2020 13:18
Last Modified : 18 Feb 2020 09:57
URI: http://epubs.surrey.ac.uk/id/eprint/853394

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