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Mycobacterium bovis uses the ESX-1 Type VII secretion system to escape predation by the soil-dwelling amoeba Dictyostelium discoideum

Butler, Rachel E., Smith, Alex A., Mendum, Tom A., Chandran, Aneesh, Wu, Huihai, Lefrançois, Louise, Chambers, Mark, Soldati, Thierry and Stewart, Graham R. (2020) Mycobacterium bovis uses the ESX-1 Type VII secretion system to escape predation by the soil-dwelling amoeba Dictyostelium discoideum The ISME Journal.

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Abstract

Mycobacterium bovis is the causative agent of bovine tuberculosis and the predominant cause of zoonotic tuberculosis in people. Bovine tuberculosis occurs in farmed cattle but also in a variety of wild animals, which form a reservoir of infection. Although direct transmission of tuberculosis occurs between mammals, the low frequency of contact between different host species and abundant shedding of bacilli by infected animals suggests an infectious route via environmental contamination. Other intracellular pathogens that transmit via the environment deploy strategies to survive or exploit predation by environmental amoebae. To explore if M. bovis has this capability, we investigated its interactions with the soil and dung-dwelling amoeba, Dictyostelium discoideum. We demonstrated that M. bovis evades phagocytosis and destruction by D. discoideum and actively transits through the amoeba using the ESX-1 Type VII Secretion System as part of a programme of mechanisms, many of which have been co-opted as virulence factors in the mammalian host. This capacity of M. bovis to utilise an environmental stage between mammalian hosts may enhance its transmissibility. In addition, our data provide molecular evidence to support an evolutionary role for amoebae as training grounds for the pathogenic M. tuberculosis complex.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Butler, Rachel E.R.Shrimpton@surrey.ac.uk
Smith, Alex A.alex.smith@surrey.ac.uk
Mendum, Tom A.T.Mendum@surrey.ac.uk
Chandran, Aneeshaneesh.chandran@surrey.ac.uk
Wu, HuihaiH.Wu@surrey.ac.uk
Lefrançois, Louise
Chambers, Markm.chambers@surrey.ac.uk
Soldati, Thierry
Stewart, Graham R.G.Stewart@surrey.ac.uk
Date : 2 January 2020
Funders : National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
DOI : 10.1038/s41396-019-0572-z
Copyright Disclaimer : © The Author(s) 2020. This article is published with open access Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons. org/licenses/by/4.0/.
Depositing User : Diane Maxfield
Date Deposited : 22 Jan 2020 15:20
Last Modified : 03 Jun 2020 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/853368

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