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Chemoradiotherapy screening in a novel biomimetic polymer based pancreatic cancer model

Gupta, Priyanka, Totti, Stella, Pérez-Mancera, Pedro A., Dyke, Eleanor, Nisbet, Andrew, Schettino, Giuseppe, Webb, Roger and Velliou, Eirini (2019) Chemoradiotherapy screening in a novel biomimetic polymer based pancreatic cancer model RSC Advances, 9 (71). pp. 41649-41663.

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Abstract

Pancreatic Ductal Adenocarcinoma (PDAC) is a deadly and aggressive disease with a very low survival rate. This is partly due to the resistance of the disease to currently available treatment options. Herein, we report for the first time the use of a novel polyurethane scaffold based PDAC model for screening the short and relatively long term (1 and 17 days post-treatment) responses of chemotherapy, radiotherapy and their combination. We show a dose dependent cell viability reduction and apoptosis induction for both chemotherapy and radiotherapy. Furthermore, we observe a change in the impact of the treatment depending on the time-frame, especially for radiation for which the PDAC scaffolds showed resistance after 1 day but responded more 17 days post-treatment. This is the first study to report a viable PDAC culture in a scaffold for more than 2 months and the first to perform long-term (17 days) post-treatment observations in vitro. This is particularly important as a longer time-frame is much closer to animal studies and to patient treatment regimes, highlighting that our scaffold system has great potential to be used as an animal free model for screening of PDAC.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Gupta, Priyankapriyanka.gupta@surrey.ac.uk
Totti, Stellastyliani.totti@surrey.ac.uk
Pérez-Mancera, Pedro A.
Dyke, Eleanor
Nisbet, Andrew
Schettino, Giuseppe
Webb, Roger
Velliou, Eirinie.velliou@surrey.ac.uk
Date : 17 December 2019
Funders : IAA-EPSRC - Engineering and Physical Sciences Research Council
DOI : 10.1039/C9RA09123H
Copyright Disclaimer : Copyright 2019 The Authors. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.
Depositing User : Diane Maxfield
Date Deposited : 20 Jan 2020 10:30
Last Modified : 20 Jan 2020 10:30
URI: http://epubs.surrey.ac.uk/id/eprint/853344

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