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Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation

Hosmillo, Myra, Lu, Jia, McAllaster, Michael R, Eaglesham, James B, Wang, Xinjie, Emmott, Edward, Domingues, Patricia, Chaudhry, Yasmin, Fitzmaurice, Tim J, Tung, Matthew KH , Panas, Marc Dominik, McInerney, Gerald, Locker, Nicolas, Wilen, Craig B and Goodfellow, Ian G (2019) Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation eLife, 8, e46681. pp. 1-35.

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Abstract

Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The core stress granule component G3BP1 was identified as a host factor essential for efficient human and murine norovirus infection, demonstrating a conserved function across the Norovirus genus. Furthermore, we show that G3BP1 functions in the novel paradigm of viral VPg-dependent translation initiation, contributing to the assembly of translation complexes on the VPg-linked viral positive sense RNA genome by facilitating ribosome recruitment. Our data uncovers a novel function for G3BP1 in the life cycle of positive sense RNA viruses and identifies the first host factor with pan-norovirus pro-viral activity.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Hosmillo, Myra
Lu, Jia
McAllaster, Michael R
Eaglesham, James B
Wang, Xinjie
Emmott, Edward
Domingues, Patricia
Chaudhry, Yasmin
Fitzmaurice, Tim J
Tung, Matthew KH
Panas, Marc Dominik
McInerney, Gerald
Locker, NicolasN.Locker@surrey.ac.uk
Wilen, Craig B
Goodfellow, Ian G
Date : 11 September 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.7554/eLife.46681
Copyright Disclaimer : © 2019, Hosmillo et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Depositing User : Clive Harris
Date Deposited : 30 Oct 2019 12:25
Last Modified : 30 Oct 2019 12:25
URI: http://epubs.surrey.ac.uk/id/eprint/852992

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