University of Surrey

Test tubes in the lab Research in the ATI Dance Research

How does polyunsaturated fatty acid biosynthesis regulate T lymphocyte function?

Fielding, Barbara A, Calder, Philip C, Irvine, Nicola A, Miles, Elizabeth A, Lillycrop, Karen A, von Gerichten, Johanna and Burdge, Graham C (2019) How does polyunsaturated fatty acid biosynthesis regulate T lymphocyte function? Nutrition Bulletin, 44 (4). pp. 350-355.

[img]
Preview
Text
Fielding_et_al-2019-Nutrition_Bulletin.pdf - Version of Record
Available under License Creative Commons Attribution.

Download (168kB) | Preview
[img] Text
How does polyunsaturated fatty acid biosynthesis regulate T lymphocyte function.pdf - Accepted version Manuscript
Restricted to Repository staff only

Download (232kB)
[img] Image (Figure 1)
BNF Fig 1.tif - Supplemental Material
Restricted to Repository staff only

Download (1MB)
[img] Image (Figure 2)
BNF Fig 2.tif - Supplemental Material
Restricted to Repository staff only

Download (1MB)

Abstract

mpaired regulation of immune function characterised by chronic inflammation together with a declining protective immune response is a major challenge to healthy ageing. It is therefore important to understand the mechanisms that regulate immune function and the impact of ageing upon such processes. Appropriate induction and resolution of the immune response requires adequate availability of polyunsaturated fatty acids (PUFAs) for incorporation into cell membranes. However, humans are unable to synthesise PUFAs de novo and are dependent upon dietary intake for pre-formed PUFAs or synthesis by the liver from the essential fatty acids, linoleic acid (LA, 18:2n-6) and alpha-linolenic acid (aLNA, 18:3n-3). We have shown that activation of peripheral blood mononuclear cells (PBMCs) 37 increases PUFA biosynthesis from essential fatty acids via a mechanism that involves altered epigenetic regulation of a key gene in the pathway. Moreover, induction of PUFA synthesis is directly involved in the regulation of lymphocyte activation and proliferation. The aim of the BBSRC responsive mode award ‘How does polyunsaturated fatty acid biosynthesis regulate T lymphocyte function?’ is to determine how PUFA biosynthesis regulates T cell function and the effect of ageing on this process. The project will identify points of regulation in the biosynthetic pathway and how these might influence the capacity for up-regulation of PUFA synthesis in older individuals. We will use stable isotope tracers of LA and aLNA to determine whether newly synthesised PUFAs are preferential substrates for synthesis of lipid mediators and whether they are involved in formation of membrane microdomains that mediate cell signalling.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Fielding, Barbara AB.Fielding@surrey.ac.uk
Calder, Philip C
Irvine, Nicola A
Miles, Elizabeth A
Lillycrop, Karen A
von Gerichten, Johannaj.vongerichten@surrey.ac.uk
Burdge, Graham C
Date : December 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.1111/nbu.12404
Copyright Disclaimer : © 2019 The Authors. Nutrition Bulletin published by John Wiley & Sons Ltd on behalf of British Nutrition Foundation. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : Epigenetics; Eicosanoids; Healthy ageing; Immune function; Polyunsaturated fatty acids; Stable isotopes
Depositing User : Clive Harris
Date Deposited : 15 Oct 2019 08:19
Last Modified : 09 Dec 2019 09:35
URI: http://epubs.surrey.ac.uk/id/eprint/852930

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800